ISOLATION AND SEROTYPIC CHARACTERIZATION OF HUMAN AND ANIMAL ROTAVIRUSES

牛轮状病毒B223株为VP 7血清型 10，牛轮状病毒678株显示属于VP 7血清型8， 人轮状病毒株PA 151和PA 169被发现共享 与牛轮状病毒VP 7血清型6毒株的中和特异性。 使用人VP 7基因置换产生五个单一VP 7基因置换抑制剂。 轮状病毒株ST 3（VP 7血清型4）和牛轮状病毒株UK， NCDV、B223、678或J2538，它们中的每一个仅具有来自ST 3的VP 7基因， 菌株，其余10个基因来自牛菌株。 通过交叉- 中和试验，存在至少四种不同的牛 证实了轮状病毒VP 4血清型。 单基因替换 在猪轮状病毒Gottfried株（VP 7 血清型4）和人轮状病毒株D（VP 7血清型1）、DS-1（VP 7 血清型2）或M（VP 7血清型3）。 这些抑制剂各自包含 来自Gottfried菌株的10个基因和来自Gottfried菌株的编码VP 7的单个基因， 人类轮状病毒株。 三种猪x人免疫缺陷病毒 以及亲本戈特弗里德病毒已经成功地适应了生长 在DBS-FRhL-2二倍体细胞中。

The bovine rotavirus B223 strain was determined to belong to VP7 serotype 10, the bovine rotavirus 678 strain was shown to belong to VP7 serotype 8, and the human rotavirus strains PA151 and PA169 were found to share neutralization specificity with bovine rotavirus VP7 serotype 6 strains. Five single VP7 gene substitution reassortants were generated using a human rotavirus strain ST3 (VP7 serotype 4) and bovine rotavirus strains UK, NCDV, B223, 678, or J2538, each of which had only the VP7 gene from the ST3 strain and the remaining 10 genes from the bovine strain. By cross- neutralization assay, the existence of at least four distinct bovine rotavirus VP4 serotypes was demonstrated. Single gene substitution reassortants were generated between porcine rotavirus strain Gottfried (VP7 serotype 4) and human rotavirus strains D (VP7 serotype 1), DS-1 (VP7 serotype 2), or M (VP7 serotype 3). These reassortants were each comprised of ten genes from the Gottfried strain and a single gene encoding VP7 from the human rotavirus strain. The three porcine x human reassortant viruses as well as parental Gottfried virus have successfully been adapted to grow in DBS-FRhL-2 diploid cells.