ISOLATION AND SEROTYPIC CHARACTERIZATION OF HUMAN AND ANIMAL ROTAVIRUSES

牛轮状病毒b223株被确定为vp7血清型。 10，牛轮状病毒678株属于VP7 8型， 而人类轮状病毒株PA151和PA169被发现是相同的 与牛轮状病毒VP7血清6型毒株的中和特异性。 五个单一的VP7基因替换重组子是用一个人 轮状病毒ST3株(VP7血清4型)和牛轮状病毒UK株， NCDV、B223、678或J2538，每一个都只有ST3的VP7基因 菌株和来自牛菌株的其余10个基因。通过交叉- 中和试验，至少存在四种不同的牛 证实了轮状病毒VP4的血清型。单基因替代 猪轮状病毒戈特弗里德株(VP7)间产生重组子 血清型4)和人轮状病毒D株(VP7血清型1)、DS-1(VP7 血清型2)或M(VP7血清型3)。这些重新组合的每一个都由 戈特弗里德毒株的10个基因和编码VP7的单个基因 人类轮状病毒株。三种猪x人重配病毒 以及亲代戈特弗里德病毒成功地适应了生长 在DBS-FRhL-2二倍体细胞中。

The bovine rotavirus B223 strain was determined to belong to VP7 serotype 10, the bovine rotavirus 678 strain was shown to belong to VP7 serotype 8, and the human rotavirus strains PA151 and PA169 were found to share neutralization specificity with bovine rotavirus VP7 serotype 6 strains. Five single VP7 gene substitution reassortants were generated using a human rotavirus strain ST3 (VP7 serotype 4) and bovine rotavirus strains UK, NCDV, B223, 678, or J2538, each of which had only the VP7 gene from the ST3 strain and the remaining 10 genes from the bovine strain. By cross- neutralization assay, the existence of at least four distinct bovine rotavirus VP4 serotypes was demonstrated. Single gene substitution reassortants were generated between porcine rotavirus strain Gottfried (VP7 serotype 4) and human rotavirus strains D (VP7 serotype 1), DS-1 (VP7 serotype 2), or M (VP7 serotype 3). These reassortants were each comprised of ten genes from the Gottfried strain and a single gene encoding VP7 from the human rotavirus strain. The three porcine x human reassortant viruses as well as parental Gottfried virus have successfully been adapted to grow in DBS-FRhL-2 diploid cells.