ACTIVE SITE TARGETING OF PROTEIN TYROSINE PHOSPHATASES BY SYNCH CRYSTALLOGRAPHY

通过同步晶体学靶向蛋白质酪氨酸磷酸酶的活性位点

• 批准号: 6205794
• 负责人: STEVE ALMO
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205794
• 关键词: biological products; biomedical resource; enzymes; spectrometry; synchrotrons

RNA 3'-terminal phosphate cyclase catalyzes the ATP-dependent conversion of the 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. It is an essential enzyme and conserved across all kingdoms. Data were collected for a crystal of RNA 3' Phosphate cyclase soaked in the solution containing 10mM AMP. Data collection statistics: 97% completeness at 3 A resolution and the R-factor (20 A - 3A) is 5.7. Unfortunately, it does not appear that AMP was bound with detectable occupancy.

RNA 3'-末端磷酸环化酶催化 ATP 依赖性 3'-磷酸酯转化为2',3'-环状磷酸二酯 RNA 末端。 它是一种必需的酶，在所有酶中都是保守的 王国。 收集 RNA 3' 磷酸盐晶体的数据 环化酶浸泡在含有10mM AMP的溶液中。 数据收集 统计数据：3 A 分辨率和 R 因子（20 A - 3A) 为 5.7。 不幸的是，AMP 似乎没有被绑定 具有可检测的占用率。