HYPOHXANTHINE GUANINE PHOSPHORIBOSYL TRANSFERASE: FOR NEW MALARIA CHEMOTHERAPIES

惠普(7，8-dihydro-6-hydroxymethylpterin-pyrophosphokinase)拥有 在大肠杆菌中得到了表达。它是叶酸中的第一种酶 综合。与哺乳动物细胞不同，哺乳动物细胞从 在饮食中，大多数微生物必须从头合成叶酸，因为 它们缺乏运输途径。因此，HPPK非常重要 作为选择性抗微生物化疗的潜在靶点。数据 以1.6E分辨率(SPACE)收集了天然蛋白质 P21组，a=36.3E，b=51.9E，c=41.2E，=110.3，RSYM=4.4%)。其他 收集的数据：HPPK/MgATP复合体：1.6的单波长数据 A.HPPK/HP复合体：2.1A的单波长数据。

7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase (HPPK) has been expressed from E. coli. It is the first enzyme in folate synthesis. Unlike mammalian cells that take up folate cofactors from the diet, most microorganisms must synthesize folates de novo, since they lack the transport pathway. For this reason, HPPK is important as a potential target for selective antimicrobial chemotherapy. Data for native protein have been collected at 1.6 E resolution (space group P21, a=36.3 E, b=51.9 E, c=41.2 E, =110.3 , Rsym=4.4%). Other data collected: HPPK / MgATP complex: single wavelength data at 1.6 A. HPPK / HP complex: single wavelength data at 2.1 A.