SYNCHROTRON CRYSTALLOGRAPHY BEAMLINE X9A STRUCTURE FUNCTION STUDIES

同步加速器晶体学光束线 X9A 结构功能研究

• 批准号: 6120411
• 负责人: STEVE ALMO
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120411
• 关键词: biological products; biomedical resource; building /facility design /renovation; enzymes; lipids; nucleic acids; proteins; spectrometry

Specific structural projects that will benefit from this facility, due to weakly diffracting crystals or difficulty in obtaining multiple derivatives, include the accessory proteins regulating eukaryotic RNA polymerase, components of the eukaryotic DNA polymerase system, E. coli RNA polymerase, regulation and structure of ion channels, proteins that crosslink, bundle and sever actin filaments, SH2 and SH3 modules, phosphatases, cyclin-dependent kinases and acyl carrier protein-dependent acyl transferases. These structural results will have major impact on areas of central importance in cell biology, including eukaryotic and prokaryotic transcriptional control, signal transduction, eukaryotic DNA replication, prokaryotic cell wall biosynthesis, regulation of the actin cytoskeleton, mechanisms of membrane protein function and cell cycle control. These structural results will also directly benefit the programs of our immediate collaborators, and, most importantly will provide the structural foundation that is essential for future work by the large number of biologists working in each of these sub-disciplines.

将受益于该设施的具体结构项目， 由于弱衍射晶体或难以获得多个 衍生物，包括调节真核RNA的辅助蛋白 聚合酶、真核生物DNA聚合酶系统的组分、E. coli RNA聚合酶，离子通道的调控和结构， 交联、捆绑和切断肌动蛋白丝的蛋白质，SH 2和SH 3 模块、磷酸酶、细胞周期蛋白依赖性激酶和酰基载体 蛋白质依赖性酰基转移酶。 这些结构性成果将 对细胞生物学中的核心重要领域产生重大影响， 包括真核和原核的转录控制、信号转导 转导，真核DNA复制，原核细胞壁 生物合成，肌动蛋白细胞骨架的调节， 膜蛋白功能和细胞周期控制。 这些结构 结果也将直接有利于我们的方案， 合作者，而且，最重要的是将提供结构 这是一个基础，对今后的工作至关重要， 生物学家在这些分支学科中工作。