STRUCT OF EVH1, TARGETING MODULE FOR SITE SPECIFIC ACTIN FILAMENT ASSEMBLY

EVH1 的结构，位点特异性肌动蛋白丝组装的靶向模块

• 批准号: 6205800
• 负责人: STEVE ALMO
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6205800
• 关键词: biological products; biomedical resource; enzymes; proteins; spectrometry; synchrotrons

Frozen TE2-SeMet crystals diffracted to ~2 A. Fluorescence scan has accurately determined the wavelengths for data collection. Three data sets have been collected from one seleno-methionine labeled crystal at 2.5A resolution at selected wavelengths. The exposure time was 45 seconds/frame and with 1 degree oscillations. The data are of very good quality with internal R-factors ranging from 4.3% to 4.8% and the overall completeness is 99.7%. Preliminary scaling results suggest that the signal should be strong enough to solve the structure using Se atoms as the anomalous scatterers. Experiments using direct methods as well as the Patterson function are carried out to find the positions of the Se atoms and calculate the phases.

冻结的Te_2-SeMet晶体衍射到~2 A荧光扫描 已经准确地确定了数据收集的波长。三 数据集是从一个标记为硒蛋氨酸的 晶体在选定的波长下，分辨率为2.5A。曝光时间 是45秒/帧，并有1度振荡。这些数据是 质量非常好，内部R系数在4.3%到4.8%之间 总体完备率为99.7%。初步定标结果 建议信号应该足够强，可以解决结构问题 使用Se原子作为反常散射体。使用DIRECT的实验 方法以及Patterson函数被用来寻找 计算了Se原子的位置，并计算了相组成。