NMR ANALYSIS OF RIESKE PROTEIN IRON SULFUR CLUSTER & SURROUNDING RESIDUES

Rieske蛋白铁硫簇的核磁共振分析

• 批准号: 6120932
• 负责人: BRIAN G FOX
• 金额: $ 1.08万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120932
• 关键词: biological products; biomaterials; biomedical resource; environmental health; nuclear magnetic resonance spectroscopy; proteins

?9 desaturase (?9D) introduces a cis double bond at the 9, 10 position of stearoyl-ACP to form olelyl-ACP. Preliminary spectroscopic studies indicate the enzyme contains a novel oxo- or hydroxo-bridged diiron clusters. 1H NMR shall be used to characterize the paramagnetically shifted resonances associated with the oxidized and reduced iron centers. Further, the temperature dependance of the chemical shifts will be examined to determine if the ferrous ions are anti-ferromagnetically coupled.

?9 去饱和酶 (?9D) 在 9、10 处引入顺式双键 硬脂酰-ACP的位置形成油酰-ACP。 初步的 光谱研究表明该酶含有一种新型的氧代或 羟基桥接的二铁簇。 1H NMR 应用于表征 与氧化相关的顺磁位移共振 和还原铁中心。 此外，温度依赖性 将检查化学位移以确定亚铁离子是否 反铁磁耦合。