FLUORESCENCE EEM SPECTROSCOPY FOR DETECTION OF DYSPLASIA IN EPITHELIAL TISSUES

我们开发了体内EEM系统，可以收集10 用 10 种不同光源激发的荧光光谱 同一组织的漫反射光谱在不到 第二。 该系统目前正用于研究癌前病变 巴雷特食道和结肠的变化。 到目前为止我们已经检查了 近 55 名巴雷特氏病患者正在接受发育不良监测 食道。 该系统也被用于布莱根妇女医院 研究结肠腺瘤性息肉和发育不良的医院。 的 快速 EEM 系统提供的丰富荧光信息 使用基于模型的方法和主成分进行分析 分析方法。

We have developed in vivo EEM system which can collect 10 fluorescence spectra excited with 10 different light sources and diffuse reflectance spectra from the same tissue in less than a second. This system is currently being used to study precancerous changes in Barrett's esophagus and colon. So far we have examined nearly 55 patients undergoing surveillance for dysplasia in Barrett's esophagus. The system is also being used at the Brigham and Women's Hospital for studying adenomatous polyps and dysplasia in colon. The wealth of fluorescence information provided by rapid EEM system is being analyzed using model based approach and principal component analysis approach.