COMBINED RAMAN & FLUORESCENCE METHODS FOR BREAST CANCER DETECTION & IMAGING

我们正在开发两种基于近红外拉曼的光学方法 乳腺癌的光谱学和荧光光子迁移 诊断和成像。拉曼光谱，一种原位测量技术 组织化学分析，可以通过光纤针进行 作为针吸或芯的一部分插入乳房的探头 活组织检查。荧光光子迁移正在被开发为一种 用于定位病变的光学层析成像技术。我们设想 这两种技术可以与荧光成像技术相结合 首先用于定位可疑病变，以及拉曼信息， 通过将光纤探头穿过活检针，然后 用于准确诊断。在本年度，我们演示了 拉曼光谱准确区分正常、良性和恶性的能力 组织通过利用830 nm的光。我们还展示了光子 迁移成像可以用来精确地定位小荧光 用逼真的光学技术嵌入到厚真实介质中的物体 属性，从而展示了该技术在 光学成像。

We are developing two optical methods based on near infrared Raman spectroscopy and fluorescence photon migration for breast cancer diagnosis and imaging. Raman spectroscopy, an in situ technique for histochemical analysis, can be implemented via a fiber optic needle probe inserted into breast as part of needle aspiration or core biopsy. Fluorescence photon migration is being developed as an optical tomographic technique for localizing lesions. We envision that the two techniques can be integrated, with fluorescence imaging first to used to localize a suspicious lesion, and Raman information, obtained by passing a fiber optic probe through a biopsy needle, then used for accurate diagnosis. In the current year, we demonstrated the ability of Raman to classify accurately normal, benign, and malignant tissues by utilizing 830 nm light. We also showed that photon migration imaging can be used to localize accurately small fluorescent objects imbedded in a thick trubid m edium with realistic optical properties, thus demonstrating the potential of this technique for optical imaging.