PURCHASE OF MICRO LC/NANO ESIQQTOF MASS SPECTROMETER

购买微型 LC/NANO ESIQQTOF 质谱仪

• 批准号: 6291383
• 负责人: ALMA L BURLINGAME
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2005-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291383
• 关键词: biomedical equipment purchase; mass spectrometry

This proposal requests funds for the purchase of a new microcapillary HPLC nanoflow rate electrospray tandem quadrupole orthogonal- acceleration time-of-flight mass spectrometer (Q-STAR). This shared instrument will be used for structural studies of bio-macromolecules and their myriad covalent modifications that are of critical importance to on- going research in the biological and medical sciences in the San Francisco Bay Area. It will be housed in the NIH NCRR-supported Mass Spectrometry Resource at UCSF. This environment ensures the availability of the scientific, technical and management expertise required to optimize its productivity in solving problems at the forefront of macromolecular biology and medicine for a large number of investigators. The proposed instrumentation will provide high precision, accurate mass CID spectra at high absolute sensitivity from on-line microcapillary separations and sequence analyses of protein digests at the femtomole level. This powerful capability is currently unavailable to these investigators. The major users are a group of 17 scientists and clinicians involved in over 31 NIH-supported research programs. These investigators have established needs for protein identification and identification of phosphorylation sites and other protein modifications that will be met with the proposed new instrument system These research projects are at the forefront of biomedical problems involving natural and aberrant proteins required in deciphering cell function and dysfunction, respectively. Most of these studies require the high absolute sensitivity and precision performance of the instrument requested. Prof. Keith Yamamoto will chair the Advisory Committee responsible for the overall guidance of the shared instrument's use. The committee will assure that the time is shared equitably and that some 10% of its time will be made available to other projects requiring its unique capability. The UCSF Academic Senate's Committee on Research and the primary users will contribute $45,162 toward this purchase. Mr. David Maltby, who has extensive experience in electrospray and other types of biological mass spectrometry, will oversee scheduling, training and maintenance and will provide daily supervision of usage. Long term productive usage and maintenance will be assured by the close association with a major Mass Spectrometry Facility and financial support of the major users.

该提案请求资金购买新型微毛细管 HPLC 纳流速率电喷雾串联四极杆正交加速飞行时间质谱仪 (Q-STAR)。该共享仪器将用于生物大分子及其无数共价修饰的结构研究，这对于旧金山湾区正在进行的生物和医学科学研究至关重要。它将存放在 NIH NCRR 支持的 UCSF 质谱资源中。这种环境确保了大量研究人员能够获得所需的科学、技术和管理专业知识，以优化其解决大分子生物学和医学前沿问题的生产力。所提出的仪器将通过在线微毛细管分离和飞摩尔水平的蛋白质消化序列分析，以高绝对灵敏度提供高精度、准确的质量 CID 谱。这些研究人员目前无法使用这种强大的功能。主要用户是由 17 名科学家和临床医生组成的团体，参与超过 31 个 NIH 支持的研究项目。这些研究人员已经确定了蛋白质鉴定和磷酸化位点鉴定以及其他蛋白质修饰的需求，这些需求将通过拟议的新仪器系统来满足。这些研究项目处于生物医学问题的前沿，涉及分别破译细胞功能和功能障碍所需的天然和异常蛋白质。大多数这些研究都需要所要求的仪器具有高绝对灵敏度和精确性能。 Keith Yamamoto 教授将担任咨询委员会主席，负责共享仪器使用的总体指导。委员会将确保公平地分配时间，并将大约 10% 的时间提供给需要其独特功能的其他项目。加州大学旧金山分校学术参议院研究委员会和主要用户将为此次购买捐赠 45,162 美元。 David Maltby 先生在电喷雾和其他类型的生物质谱分析方面拥有丰富的经验，他将监督调度、培训和维护，并提供日常使用监督。与主要质谱设施的密切联系以及主要用户的财政支持将保证长期的生产使用和维护。