PURCHASE OF MICRO LC/NANO ESIQQTOF MASS SPECTROMETER

该提案要求提供资金，用于购买一台新的微毛细管高效液相色谱纳米流电喷雾串联四极正交加速飞行时间质谱仪(Q-STAR)。这一共享仪器将用于生物大分子及其无数共价修饰的结构研究，这些修饰对旧金山湾区正在进行的生物和医学科学研究至关重要。它将被安置在加州大学旧金山分校NIH NCRR支持的质谱学资源中。这种环境确保了所需的科学、技术和管理专业知识的可获得性，以优化其生产力，为大量研究人员解决大分子生物学和医学的前沿问题。该仪器将通过在线微毛细管分离和飞摩尔水平的蛋白质消化序列分析，以高绝对灵敏度提供高精度、准确的质量CID光谱。这些调查人员目前无法使用这种强大的能力。主要用户是一个由17名科学家和临床医生组成的小组，他们参与了NIH支持的31个研究项目。这些研究人员已经确定了蛋白质鉴定和磷酸化位点的鉴定以及拟议的新仪器系统将满足的其他蛋白质修饰的需求。这些研究项目分别处于生物医学问题的前沿，涉及破译细胞功能和功能障碍所需的天然和异常蛋白质。这些研究中的大多数都需要所需仪器的高绝对灵敏度和精度性能。山本教授将担任咨询委员会主席，负责对共享仪器的使用提供全面指导。委员会将确保公平地分配时间，并将大约10%的时间用于需要其独特能力的其他项目。加州大学旧金山分校学术参议院研究委员会和主要用户将为此次购买贡献45,162美元。David Maltby先生在电喷雾和其他类型的生物质谱学方面拥有丰富的经验，他将负责计划、培训和维护，并将提供日常使用监督。长期的生产性使用和维护将得到与主要质谱学设施的密切联系和主要用户的财政支持的保证。

This proposal requests funds for the purchase of a new microcapillary HPLC nanoflow rate electrospray tandem quadrupole orthogonal- acceleration time-of-flight mass spectrometer (Q-STAR). This shared instrument will be used for structural studies of bio-macromolecules and their myriad covalent modifications that are of critical importance to on- going research in the biological and medical sciences in the San Francisco Bay Area. It will be housed in the NIH NCRR-supported Mass Spectrometry Resource at UCSF. This environment ensures the availability of the scientific, technical and management expertise required to optimize its productivity in solving problems at the forefront of macromolecular biology and medicine for a large number of investigators. The proposed instrumentation will provide high precision, accurate mass CID spectra at high absolute sensitivity from on-line microcapillary separations and sequence analyses of protein digests at the femtomole level. This powerful capability is currently unavailable to these investigators. The major users are a group of 17 scientists and clinicians involved in over 31 NIH-supported research programs. These investigators have established needs for protein identification and identification of phosphorylation sites and other protein modifications that will be met with the proposed new instrument system These research projects are at the forefront of biomedical problems involving natural and aberrant proteins required in deciphering cell function and dysfunction, respectively. Most of these studies require the high absolute sensitivity and precision performance of the instrument requested. Prof. Keith Yamamoto will chair the Advisory Committee responsible for the overall guidance of the shared instrument's use. The committee will assure that the time is shared equitably and that some 10% of its time will be made available to other projects requiring its unique capability. The UCSF Academic Senate's Committee on Research and the primary users will contribute $45,162 toward this purchase. Mr. David Maltby, who has extensive experience in electrospray and other types of biological mass spectrometry, will oversee scheduling, training and maintenance and will provide daily supervision of usage. Long term productive usage and maintenance will be assured by the close association with a major Mass Spectrometry Facility and financial support of the major users.