HIGH RES DATA COLLECTION ON NATIVE & COMPLEXED SULFITE REDUCTASE HEMOPROTEIN

原生高分辨率数据收集

• 批准号: 6437471
• 负责人: BRIAN R CRANE
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6437471
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Sulfite Reductase Hemoprotein (SiRHP) constitutes the catalytic subunit of the multicomponet enzyme complex Sulfite Reductase. Sulfite Reductase is responsible for the six electron reduction of sulfite to sulfide in the biological assimilation of sulfur. When supplied with artificial electron donors, SiRHP, a 64 KDa monomer, is fully capable of the reductive chemistry performed by the complex. The hemoprotein subunit utilizes a unique assembly of cofactors to transfer electrons to substrate. The structure of the oxidized enzyme is currently being built and refined. Atomic resolution information on this enzyme in its various complexed and reduced forms should lend great insight into the chemistry involved in the biological reduction of low molecular weight compounds.

亚硫酸盐还原酶血红素蛋白（SiRHP）构成了催化 亚单位的多组分酶复合亚硫酸盐还原酶。 亚硫酸盐还原酶负责六电子还原 在硫的生物同化作用中，亚硫酸盐转化为硫化物。 当 提供人工电子供体，SiRHP，64 KDa单体， 完全能够由络合物进行还原化学。 血红素蛋白亚基利用辅因子的独特组装， 将电子转移到衬底上。 氧化酶的结构 目前正在建设和完善中。 原子分辨率信息 这种酶在其各种复杂的和还原的形式应该借给 对生物还原中所涉及的化学过程的深刻见解 低分子量化合物。