MANNOSIDASE II FROM INSECT CELL HOST FOR BACULOVIRUS EXPRESSION, SF9 CELLS

来自昆虫细胞宿主的甘露糖苷酶 II 用于表达杆状病毒，SF9 细胞

• 批准号: 6653556
• 负责人: KELLEY W. MOREMEN
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653556
• 关键词: AIDS; Mammalia; biomedical resource; immunology; infection; inflammation; lymphatic system; plants; respiratory system; structural biology; virus

Pneumocystis carinii is a prevalent opportunistic fungal infection striking HIV-afflicted hosts. The cell wall of P. carinii is proving to be structurally more complex than previously thought. We have purified, by preparatory SDS-PAGE, a family of glycoproteins from P. carinii cell wall material isolated by Zymolyase [?(1.3)-glucanase] treatment. We are in the process of characterizing these cell wall glycoproteins with a number of MS-based approaches.

卡氏肺孢子虫是一种常见的机会性真菌感染。 袭击了感染艾滋病毒的宿主。卡氏肺孢子虫的细胞壁证明 在结构上比之前想象的更复杂。我们有 通过预备性SDS-PAGE分离纯化了一个糖蛋白家族。 发酵酶分离Carinii细胞壁材料[？(1.3)-葡聚糖酶] 治疗。我们正在研究这些细胞壁的特征 糖蛋白与一些基于MS的方法。