课题基金 / 基金详情

STAFF PRIORITY TIME STRUCTURAL STUDIES OF MOFE CONTAINING PART OF NITROGENASE

STAFF PRIORITY TIME STRUCTURAL STUDIES OF MOFE CONTAINING PART OF NITROGENASE
工作人员优先对含有部分固氮酶的 MOFE 进行时间结构研究
批准号：
6586497
负责人：
S M SOLTIS
金额：
$14.32万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
项目状态：
已结题
起止时间：
2002-03-01 至 2003-02-28
来源：
https://reporter.nih.gov/project-details/6586497
关键词：
bacteria bioimaging /biomedical imaging biomaterials biomedical resource proteins radiography structural biology technology /technique

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英文摘要
Crystallographic data were recorded from the MoFe containing part of nitrogenase to 1.5 E resolution. Additional data sets were collected where the samples were pressurized with Xe, CO and NO, respectively. Preliminary indications suggested that subtle changes in the oxidation state of the Fe-S cluster may have contributed to a misassignment of the geometry. A data set was collected from a sample in which the oxidation state was controlled by reducing the crystal in mother liquor prior to flash-cooling. The resulting maps confirmed that the previous model was incorrect and a revised model is forthcoming.
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