STAFF PRIORITY TIME STRUCTURAL STUDIES OF MOFE CONTAINING PART OF NITROGENASE

工作人员优先对含有部分固氮酶的 MOFE 进行时间结构研究

• 批准号: 6658464
• 负责人: S M SOLTIS
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658464
• 关键词: bacteria; bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology; technology /technique

Crystallographic data were recorded from the MoFe containing part of nitrogenase to 1.5 E resolution. Additional data sets were collected where the samples were pressurized with Xe, CO and NO, respectively. Preliminary indications suggested that subtle changes in the oxidation state of the Fe-S cluster may have contributed to a misassignment of the geometry. A data set was collected from a sample in which the oxidation state was controlled by reducing the crystal in mother liquor prior to flash-cooling. The resulting maps confirmed that the previous model was incorrect and a revised model is forthcoming.

从含MoFe的部分记录晶体学数据