PHYSICAL MECHANISMS OF PULSED LASER MICROBEAM INTERACTIONS WITH CELLS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。利用时间分辨明场、相对比和荧光显微镜方法，我们正在研究细胞裂解、细胞手术以及分子递送到单个细胞（光注射）或整个细胞群（光变形）的物理机制。这些时间分辨技术允许在纳秒级的时间尺度上可视化等离子体形成、冲击波传播、空化泡形成和坍缩等过程。此外，外源性荧光报告的使用使我们能够确定时间分辨细胞对微束扰动的响应。目前，对PtK2细胞系的研究主要集中在使用532 nm激光波长，脉冲持续时间在200ps - 5ns范围内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using time-resolved brightfield, phase contrast and fluorescence microscopy methods we are investigating the physical mechanisms responsible for cell lysis, cellular surgery, as well as molcular delivery to individual cells (optoinjection) or entire cell populations (optoporation). These time-resolved technqiues allow for visualization, on time scales as rapid as nanoseconds, processes such as plasma formation, shockwave propagation, cavitation bubble formation and collapse. Moreover, the use of exogenous fluorescence reporters enable us to determine the time-resolved cellular response to the microbeam perturbations. Currently, studies are focused on the PtK2 cell line using the 532 nm laser wavelength with pulse durations in the range of 200ps - 5ns.