PHYSICAL MECHANISMS OF PULSED LASER MICROBEAM INTERACTIONS WITH CELLS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using time-resolved brightfield, phase contrast and fluorescence microscopy methods we are investigating the physical mechanisms responsible for cell lysis, cellular surgery, as well as molcular delivery to individual cells (optoinjection) or entire cell populations (optoporation). These time-resolved technqiues allow for visualization, on time scales as rapid as nanoseconds, processes such as plasma formation, shockwave propagation, cavitation bubble formation and collapse. Moreover, the use of exogenous fluorescence reporters enable us to determine the time-resolved cellular response to the microbeam perturbations. Currently, studies are focused on the PtK2 cell line using the 532 nm laser wavelength with pulse durations in the range of 200ps - 5ns.