DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 2566754
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2566754
• 关键词: Giardia; genetic mapping; genetic transcription; glutamate dehydrogenase; human tissue; microorganism genetics; nucleic acid sequence; protein sequence; protein transport; protozoal antigen; reporter genes; transfection

Giardia lamblia is the most common disease-causing parasite in the United States. However, little is known about how transcription is controlled. Prior studies developed a transient transfection system which was used to study upstream elements that may be important in transcription control. The upstream region of the Giardia glutamate dehydrogenase gene was analyzed using the firefly luciferase gene as a reporter. Only 44bp were required and 3 motifs within this region were affected by transcription. One region was the AT-rich region encompassing the transcription initiation site and start codon; a second region was a CAAAT region at -34 and a third region included an AG dinucleotide at -15. A number of proteins undergoing vesicular transport or important in the process were identified using antibodies specific to Giardia transport vesicles. Partial sequences were obtained.

蓝氏贾第虫是美国最常见的致病寄生虫。 各州。然而，人们对转录是如何控制的知之甚少。 先前的研究开发了一种瞬时转染系统，该系统用于 研究在转录中可能重要的上游元素 控制力。贾第虫谷氨酸脱氢酶基因的上游区域 以萤火虫荧光素酶基因为报告基因进行了分析。仅44个基点 是必需的，该区域内的3个模体受 抄写。其中一个地区是AT丰富的地区，包括 转录起始点和起始密码子；第二个区域是 CAAAT区位于-34，第三个区包括AG二核苷酸 -15. 一些正在进行囊泡运输或在细胞内起重要作用的蛋白质 使用针对贾第虫运输的抗体来识别过程 水泡。获得了部分序列。