DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 6160594
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6160594
• 关键词: Giardia; electroporation; genetic mapping; immunofluorescence technique; microorganism genetics; nucleic acid sequence; protein sequence; protozoal antigen; pulsed field gel electrophoresis; reporter genes; surface antigens; transfection; transfection /expression vector

A transient transfection system for Giardia lamblia was previously developed. Although useful for some experiments, its usefulness was limited. A stable transfection system was developed in Giardia and employed to stably express foreign proteins. The system has great utility to study cellular biology of Giardia and understand the control of variant surface protein expression.

一种针对蓝氏贾第鞭毛虫的瞬时转基因系统 发展起来的。虽然它对一些实验有用，但它的用处是 有限的。在贾第鞭毛虫中建立了稳定的转导系统 用来稳定表达外源蛋白的。这个系统有很好的 对研究贾第鞭毛虫细胞生物学和了解其控制的作用 不同的表面蛋白表达。