DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 3790735
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3790735
• 关键词: Giardia; genetic manipulation; genetic markers; human subject; microorganism genetics; nucleic acid sequence; ribosomal DNA

Large differences among Giardia isolates were noted. 18S rDNA sequence differences were detected among isolates but were identical among the same Giardia Group. Comparison of the triosphophate isomerase genes of two isolates known to differ from each other by other criteria revealed a 19% sequence difference which resulted in a 9% amino acid divergence. A transcript was identified, sequenced and characterized. It was transcribed in only one of three Groups of Giardia and therefore is a unique marker of this group. The genomic organization of the VSP genes can differ among isolates.

贾第鞭毛虫分离株之间的差异很大。18S rDNA序列 检测到不同菌株之间的差异，但在 同样是贾迪亚集团。三磷酸腺苷异构酶基因的比较 两个已知在其他标准上彼此不同的分离株揭示了 19%的序列差异导致了9%的氨基酸差异。一个 对转录本进行鉴定、测序和鉴定。确实是 只在贾第鞭毛虫的三组中的一组中转录，因此是一个 此组的唯一标记。VSP基因的基因组组织 在不同的菌株之间可能会有所不同。