IMMUNOCHEMISTRY OF PARASITIC DISEASES

寄生虫病的免疫化学

• 批准号: 4688372
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4688372
• 关键词: Cryptosporidium; Giardia; antibody; antigen antibody reaction; chromatography; enzyme linked immunosorbent assay; filariasis; giardiasis; helminthic antigen; host organism interaction; human tissue; humoral immunity; immunochemistry; immunofluorescence technique; immunoglobulin G; immunoglobulin M; microorganism culture; parasitic diseases; protozoal antigen; radioimmunoassay; schistosomiasis; secretion; serology /serodiagnosis; surface antigens

Studies are being performed on three parasitic parasites: schistosomes, Giardia, and Cryptosporidium. Nine more isolates of Giardia have been axenized and their DNA, surface antigens, and biologic behavior are being studied. Although still incomplete, these studies, thus far, confirm the large heterogeneity noted among the previously studied 15 isolates. Monoclonal antibodies to a 170,000 Kd major surface antigen of WB and WB-like isolates were produced. These monoclonal antibodies are specific for the WB-type of isolate and are cytotoxic to these organisms. In order to study biological importance and differences between isolates, a model infection of Giardia in jirds was established. Isolates differ in ability to invoke self cure, immunity and protection to challenge with heterologous isolates. An ELISA assay to measure antibody to Crytosporidium antibodies to oocysts was developed. IgG and/or IgM antibody responses in non-AIDS patients were practically always detected. IgG antibodies were found commonly in AIDS patients. In additon, IgG antibodies to Cryptosporidium were detected frequently in patients with other parasitic diseases suggesting that infection is common. A study evaluating the use of ultrasonography in the diagnosis of Symmers' fibrosis in on-going in Khartoum, Sudan.

目前正在对三种寄生虫进行研究：寄生虫， 贾第虫和隐孢子虫。 又有九个贾第虫分离株 它们的DNA、表面抗原和生物学行为正在被 研究了 尽管这些研究还不完整，但到目前为止， 在先前研究的15个分离株中观察到较大的异质性。 抗WB的170，000 Kd主要表面抗原的单克隆抗体， 产生WB样分离物。 这些单克隆抗体是特异性的 对于WB型分离株，对这些微生物具有细胞毒性。 为了 为了研究分离株之间的生物学重要性和差异， 沙鼠贾第虫感染。 分离株的能力不同 以激发自身治愈、免疫和保护以对抗异源性攻击 分离株 一种检测隐孢子虫抗体的ELISA方法 卵囊发育。 非艾滋病患者的IgG和/或IgM抗体应答 患者几乎总是被发现的。 发现IgG抗体 常见于艾滋病患者。 此外，隐孢子虫IgG抗体 常见于其他寄生虫病患者 说明感染很常见 一项评估使用 超声在诊断肝纤维化中的应用 苏丹，喀土穆。