IMMUNOCHEMISTRY OF PARASITIC DISEASES

寄生虫病的免疫化学

• 批准号: 3768736
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3768736
• 关键词: Giardia; SCID mouse; aminoacid metabolism; athymic mouse; disease /disorder model; gastrointestinal infection; genetic strain; giardiasis; host organism interaction; human tissue; immunochemistry; insulinlike growth factor; laboratory mouse; microorganism culture; microorganism metabolism; parasitism; protozoal antigen; recombinant proteins

An infection model of Giardia lamblia into adult mice has been developed. Only one isolate of nine studied consistently infected adult mice. Seven mice strains were readily infected but showed different courses of infections. The course of infection, changes in expressed VSP over time, and humoral responses were noted in a number of strains of mice as well as SCID and nude mice. Large numbers of trophozoites were present in the small intestines 1-2 weeks after inoculation. This was followed by a dramatic reduction in the numbers of trophozoites in the intestines. Two of three strains remained chronically infected, while a third was cured. SCID mice failed to show a reduction in the number of intestinal trophozoites over time. This model system allows the analysis of host- parasite interactions, especially those responses important in antigenic variation, maintenance of chronic infections, and cure. The metabolism of cysteine in Giardia was studied. Giardia could neither manufacture cysteine de novo or convert other sulfur-containing compounds to cysteine. Two mechanisms appeared responsible for uptake, one of which was non-saturable. Oxidation of cysteine to cystine occurs readily in culture and can be prevented by the use of a Cu++ chelating compound, batocuproine. ICF II, a component of Cohn fraction IV-1, is an essential growth- promoting factor of Giardia. Recombinant protein supported growth and cysteine uptake in the absence of serum, a necessary component for culture.

已开发出兰氏贾第鞭毛虫感染成年小鼠的模型。 在九只研究中，只有一只分离株持续感染成年小鼠。 七 小鼠品系很容易被感染，但表现出不同的病程 感染。 感染过程、表达的 VSP 随着时间的变化、 在许多品系的小鼠中也发现了体液反应 如 SCID 和裸鼠。体内存在大量滋养体 接种后1-2周小肠。 随后是 肠道内滋养体数量急剧减少。 二 三种菌株仍处于慢性感染状态，而三分之一已被治愈。 SCID 小鼠未能表现出肠道数量的减少 随着时间的推移滋养体。 该模型系统允许分析主机 寄生虫相互作用，特别是那些在抗原性中重要的反应 变化、慢性感染的维持和治愈。 研究了贾第鞭毛虫中半胱氨酸的代谢。 贾第鞭毛虫都不能 从头制造半胱氨酸或转化其他含硫化合物 至半胱氨酸。 似乎有两种机制负责吸收，其中之一是 这是不饱和的。半胱氨酸容易氧化成胱氨酸 在培养物中，可以通过使用 Cu++ 螯合化合物来预防， 巴托铜灵。 ICF II 是 Cohn 组分 IV-1 的一个组成部分，是一种重要的生长- 贾第鞭毛虫的促进因子。重组蛋白支持生长和 在没有血清的情况下摄取半胱氨酸，这是 文化。