DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 5200450
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5200450
• 关键词: DNA; Entamoeba histolytica; Giardia; Trichomonas; biochemical evolution; gene expression; human tissue; microorganism classification; mitochondria; molecular biology; monoclonal antibody; nucleic acid sequence; organelles; protozoal antigen; transfection

For the first time a transient transfection system was developed for Giardia lamblia using flanking regions of the glutamate dehydrogenase gene and the luciferase reporter gene. This will enable study of transcription of genes such as those that are induced during encystment, genes which undergo antigenic variation and constitutively expressed genes. Cwp2, a second protein constituent of the Giardia lamblia cyst wall was identified and sequenced. Monoclonal antibodies were made to encystment vesicles and cyst walls and were used to identify proteins important in vesicular transport and constituents of the cyst wall. Entamoeba histolytica contains genes encoding proteins normally associated with the mitochondrion, an organelle this organism appears to lack. Sequence and phylogenetic analysis of two such Entamoeba genes has been performed and confirms the specific relatedness. This has major implications for our understanding of the unique biochemistry of this parasite. The same genes have been sought in other parasites thought to lack mitochondria. Analysis of a homologous gene isolated from Trichomonas indicates a similar evolutionary history and implies descent from a mitochondrion bearing ancestor.

首次开发了瞬时转染系统 贾第鞭毛虫利用谷氨酸脱氢酶的侧翼区域 基因和荧光素酶报告基因。这将使研究 基因的转录，例如在成囊过程中诱导的基因转录， 经历抗原变异并组成型表达的基因 基因。 Cwp2，贾第鞭毛虫囊肿的第二种蛋白质成分 墙壁被识别并排序。单克隆抗体是为了 包囊囊泡和囊壁，用于鉴定蛋白质 对囊泡运输和囊壁成分很重要。 溶组织内阿米巴含有正常编码蛋白质的基因 与线粒体相关的细胞器，该生物体似乎 缺少。 对两个这样的内阿米巴基因进行了序列和系统发育分析 已执行并确认特定相关性。这有重大 对我们理解这种独特的生物化学的影响 寄生虫。人们也在其他寄生虫中寻找相同的基因，认为 缺乏线粒体。分离自同源基因的分析 毛滴虫表明了相似的进化历史并暗示了血统 来自携带线粒体的祖先。