DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 3803162
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3803162
• 关键词: Giardia; genetic manipulation; microorganism genetics; monoclonal antibody; nucleic acid sequence; protein sequence; protozoal antigen; surface antigens

The sequences of two variant specific proteins (VSPs) have been determined. They reveal that VSPs, even from unrelated isolates, are compositionally and structurally related. Although identity among VSPs are about 50 percent they are antigenically distinct. Inexpressible epitopes are not represented in the genome. VSPs have a common 3' terminal region. If host's respond or can be made to respond to this area, vaccination may be possible. The genomic organization of VSP1267 revealed two identical genes arranged tail to tail with an intervening 3kb region. The VSP repetitive epitope recognized by Mab 6E7 in VSPWB170 is present in other isolates, but the sequences of some are different. The analogue of the G-protein ADP-ribosylation factor has been identified and sequenced in WB and GS Giardia isolates. Although there is near identity at the amino acid level, the sequences are only 87 percent identical indicating significant drift.

测定了两个变异型特异蛋白(VSP)的序列。 他们揭示了VSP，即使是来自不相关的分离株，在成分上也是 在结构上也是相关的。尽管VSP之间的身份认同约为50 它们在抗原性上是不同的。无法表达的表位不是 在基因组中有代表性。VSP有一个共同的3‘末端区域。如果 宿主应答或可使其对该区域作出应答，接种疫苗可 有可能。 VSP1267的基因组结构显示有两个相同的基因排列 尾到尾，中间有一个3kb的区域。 VSPWB170中由单抗6E7识别的VSP重复表位存在于 其他菌株，但有些菌株的序列不同。 G蛋白ADP-核糖化因子的类似物已被鉴定 并在WB和GS贾第鞭毛虫分离株中进行了测序。虽然有很近的 氨基酸水平上的同源性，序列只有87% 相同表示显著漂移。