DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 3809630
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809630
• 关键词: DNA; Giardia; cysteine; genetic manipulation; microorganism genetics; nucleic acid sequence; proteins; protozoal antigen; stress proteins; surface antigens

Analysis of two variant surface antigens from two different isolates continue. Both are cysteine-rich, contain cys-x-x-cys motifs, are highly transcribed, and are found only in the clone expressing the variant. A 170 kD cysteine-rich protein (CRP) has a repeating subunit which was found to contain the epitope which reacts with Mab 6E7. Genes have been identified which can be used to differentiate isolates. One (C4) is highly transcribed in non-WB-like Giardia and is not found in WB-like Giardia. The function of C4 is not known.

两种不同分离株表面抗原变异株的分析 继续 两者都富含半胱氨酸，含有cys-x-x-cys基序， 转录，并且仅在表达该变体的克隆中发现。 一个170 kD富含半胱氨酸蛋白（CRP）具有重复亚基， 含有与Mab 6 E7反应的表位。 基因已被确定 其可用于区分分离物。 一个（C4）是高度 在非WB样贾第虫中转录，在WB样贾第虫中不存在。 C4的功能尚不清楚。