DNA ANALYSIS OF PARASITES

寄生虫的 DNA 分析

• 批准号: 3818203
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818203
• 关键词: DNA; Giardia; endonuclease; epidemiology; genetic manipulation; genetic promoter element; gerbil /jird; giardiasis; lac operon; microorganism genetics; molecular cloning; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; parasitic disease diagnosis; protozoal antigen; surface antigens; transfection

Giardia surface antigens undergo antigenic variation. To determine the relatedness of one variant to another, cDNA libraries of two related variants were successfully produced and screened with surface specific Mab or previously defined DNA probes. Analysis and sequencing are in progress. Pulse field gradient analysis of Giardia chromosomes continue. One minor band was related to one of the major chromosomes. Markers were derived product is recognized by all infected animals and humans. Sequencing of this gene revealed no relationship to other proteins. This antigen may be valuable for detecting Giardia specific antibody responses. Attempts to transform Giardia continue. Constructs were made using a Giardia heat shock promoter and he lacZ gene and used to transform Giardia. Giardia were transformed transiently.

贾第鞭毛虫表面抗原发生抗原变异。来确定