IMMUNOCHEMISTRY OF PARASITIC DISEASES

寄生虫病的免疫化学

• 批准号: 3809560
• 负责人: T E NASH
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3809560
• 关键词: Giardia; T lymphocyte; antigen antibody reaction; antiinfective agents; antiprotozoal agents; disease /disorder model; giardiasis; humoral immunity; immunochemistry; laboratory mouse; microorganism culture; microorganism immunology; molecular cloning; monoclonal antibody; parasitic disease diagnosis; parasitic diseases; protozoal antigen; serotyping; surface antigens

The variant surface antigen repertoires and the rates these antigens are expressed differ among Giardia isolates. In cloned populations trophozoites with new variant antigens are detected every 12-13 generations in isolate WB and once every 6.5 generations in isolate GS. The ability to express variant antigens is genetically determined since some isolates lack genes encoding the surface antigens of other isolates. The variant surface antigens also differ in their ability to resist digestion with the intestinal enzymes trypsin and chymotrypsin. After exposure of sensitive clones to protease, some trophozoites remain alive and these are stably resistant to these enzymes. These antigens may be important for survival of Giardia in the intestines. Neonatal mice infected with Giardia lamblia undergo antigenic variation. Immunological studies using this model reveal T cell responses to Giardia are limited to the intestine and are not present to the variant antigen. The need for host immune responses in the successful treatment of cestode infections with praziquantel was studied in T. taeniaformis infections in the mice. Humoral factors, e.g., antibody are required for successful therapy in T. taeniaformis and probably T. solium infections as well.

变异的表面抗原库和这些抗原的比率 在贾第虫分离株中表达不同。在克隆种群滋养体中 在分离株中每12-13代检测到新的变异抗原 WB和GS每6.5代一次。表达能力 变异抗原是由基因决定的，因为一些分离株缺乏基因 编码其他分离物的表面抗原。变曲面 抗原也在它们抵抗被抗原消化的能力上不同。 肠酶胰蛋白酶和糜蛋白酶。在暴露敏感的 克隆蛋白酶，一些滋养体仍然活着，这些是稳定的 对这些酶有抵抗力。这些抗原可能是重要的生存， 肠道内有贾第虫。 感染蓝氏贾第鞭毛虫的新生小鼠发生抗原变异。 使用该模型的免疫学研究揭示了T细胞对贾第虫的反应 仅限于肠内，不存在于变体抗原中。 成功治疗绦虫需要宿主免疫应答 感染吡喹酮的T.带状疱疹感染 老鼠体液因素，例如，抗体是成功 治疗T. taeniaformis和可能的T.也会感染疟疾