MARSHALL U COBRE: GENOMICS COREP=C

马歇尔·U·科布雷：基因组学 COREP=C

• 批准号: 7720782
• 负责人: Donald Anthony Primerano
• 金额: $ 38.94万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720782
• 关键词: Area; Candidate Disease Gene; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Sequence; DNA Sequence Analysis; Data; Data Analyses; Development; Experimental Designs; Funding; Gene Expression; Gene Expression Profiling; Genomics; Goals; Grant; Human Resources; Institution; Investigation; Malignant Neoplasms; Marshal; Measurement; Messenger RNA; Methods; Molecular; Pathway interactions; Polymerase Chain Reaction; Positioning Attribute; Reagent; Research; Research Personnel; Research Project Grants; Resources; Sequence Analysis; Signal Transduction; Source; Time; Training; United States National Institutes of Health; Universities; base; data management; expectation; experience; insight; instrumentation; novel; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿

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