MARSHALL U COBRE: GENOMICS COREP=C

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。COBRE应用程序中的每个研究项目都严重依赖于基因表达分析来完成其特定目标。在此应用中最常见的基因组方法是通过微阵列进行基因表达谱分析，通过实时PCR测量单mRNA丰度，以及DNA测序和序列分析。微阵列实验和DNA测序需要昂贵的仪器和试剂，复杂的方法，以及密集的数据管理和分析。这些任务由核心设施人员最有效地执行。拟议的COBRE项目无法在没有直接获得专家基因组技术和分析支持的情况下完成。因此，该应用程序的目标是实现基因组核心设施，以满足COBRE和马歇尔大学在基于微阵列的基因表达谱，实时PCR，自动DNA测序和DNA序列分析领域的特定需求。微阵列管理人员和基因组数据分析师都需要大量的外部培训，以跟上发展的步伐，并为最终用户提供有用的分析。基因组学核心设施的长期目标是定位COBRE研究人员在癌症发展中发现新的分子途径。我们将通过追求以下两个具体目标来实现COBRE研究项目的基因组研究目标：1。实现基因组学核心设施和发展微阵列数据分析能力。基因组研究中心将由Niles博士和Primerano博士共同领导，他们在实验设计和核心管理方面具有互补的技能。鉴于联合主任的经验以及关键人员和仪器的存在，我们已经为开发Genomics核心设施做好了充分的准备。我们期望基因组学核心设施将使COBRE和马歇尔大学的研究人员能够识别关键参与细胞信号转导的候选基因，确认表达模式，并深入了解癌症分子生物学。景深害怕

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿