GENOMICS CORE

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 微阵列实验和自动化DNA测序需要复杂的方法、昂贵的仪器和试剂以及密集的数据管理和分析。基因组学核心将提供较小机构无法获得的集中服务，并提供完成生物医学研究项目所需方法的培训。我们的主要目标是实现INBRE，COBRE和WV大学和学院的其他研究项目的基因组研究目标。在WV-INBRE第二阶段，基因组学核心设施将提供以下服务： (1)基于微阵列的基因表达谱分析和数据分析的统计支持 (2)与WV-INBRE Bioinformatics Core合作进行自动化DNA测序并访问DNA序列分析软件， (3)使用实时热循环仪进行定量PCR，使用Agilent 2100生物分析仪进行DNA/RNA定量和质量评估，以及 (4)纯化和储存患者基因组DNA。 我们还将与生物信息学核心一起提供研讨会，讨论本科研究项目研究计划的核心方法。所有核心服务将在赠款期开始时提供。国家生物多样性研究所、全球生物多样性研究理事会和其他调查人员对这些服务的需求很大。 我们期望，基因组学核心设施将使INBRE和COBRE研究人员能够识别和表征参与癌症和心血管疾病的候选基因，为实验假设提供直接证据，确认基因表达模式，并深入了解候选基因的生理和生化功能。 人员 标题 D. Primerano博士 主任 J. Denvir，博士 基因组数据分析员 G. Boskovic博士 微阵列管理器 Y. Dementieva博士 生物统计学家 魏丽萍女士 基因组学核心经理 Jun Fan，博士 基因组学核心经理（7月1日至今） 杨毅，博士 生物信息学顾问 Mark Flood，博士 焦磷酸测序核心@FSU

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microarray experimentation and automated DNA sequencing require sophisticated methods, expensive instrumentation and reagents, and intense data management and analysis. The Genomics Core will provide centralized services that would not be available to smaller institutions and provide training in methods needed for completion of biomedical research projects. Our primary objective is to enable the genomic research goals of INBRE, COBRE and other research projects at WV universities and colleges. Under WV-INBRE Phase II the Genomics Core Facility will provide the following services: (1) microarray-based gene expression profiling and statistical support for data analysis (2) automated DNA sequencing and access to DNA sequence analysis software in collaboration with the WV-INBRE Bioinformatics Core, (3) access to real-time thermal cyclers for quantitative PCR and to an Agilent 2100 Bioanalyzer for DNA/RNA quantitation and quality assessment, and (4) purification and banking of patient genomic DNA. We will also provide workshops in conjunction with the Bioinformatics Core on methods central to research plans of the undergraduate research projects. All core services will be available at the start of the grant period. There is substantial need for these services among INBRE, COBRE and other investigators. It is our expectation that the Genomics Core Facility will enable INBRE and COBRE investigators to identify and characterize candidate genes involved in cancer and cardiovascular disease, provide direct evidence for experimental hypotheses, confirm patterns of gene expression, and gain insights into the physiological and biochemical functions of the candidate genes. Personnel Title D. Primerano, PhD Director J. Denvir, PhD Genomic Data Analyst G. Boskovic, PhD Microarray Manager Y. Dementieva, PhD Biostatistician Liping Wei, MS Genomics Core Manager Jun Fan, PhD Genomics Core Manager (July 1 to present) Ivana Yang, PhD Bioinformatics consultant Mark Flood, PhD Pyrosequencing Core @FSU