GENOMICS CORE

基因组核心

• 批准号: 8167680
• 负责人: Donald Anthony Primerano
• 金额: $ 12.25万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167680
• 关键词: Biochemical; Bioinformatics; Biomedical Research; Candidate Disease Gene; Cardiovascular Diseases; Centers of Research Excellence; Collaborations; Computer Retrieval of Information on Scientific Projects Database; Computer software; Core Facility; DNA; DNA Sequence; DNA Sequence Analysis; Data; Data Analyses; Doctor of Philosophy; Educational workshop; Floods; Funding; Gene Expression; Gene Expression Profiling; Genomics; Goals; Grant; Human Resources; Institution; Malignant Neoplasms; Methods; Patients; Pattern; Phase; Physiological; Reagent; Research; Research Personnel; Research Project Grants; Resources; Services; Source; Time; Training; United States National Institutes of Health; Universities; Yang; base; college; data management; expectation; insight; instrumentation

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Microarray experimentation and automated DNA sequencing require sophisticated methods, expensive instrumentation and reagents, and intense data management and analysis. The Genomics Core will provide centralized services that would not be available to smaller institutions and provide training in methods needed for completion of biomedical research projects. Our primary objective is to enable the genomic research goals of INBRE, COBRE and other research projects at WV universities and colleges. Under WV-INBRE Phase II the Genomics Core Facility will provide the following services: (1) microarray-based gene expression profiling and statistical support for data analysis (2) automated DNA sequencing and access to DNA sequence analysis software in collaboration with the WV-INBRE Bioinformatics Core, (3) access to real-time thermal cyclers for quantitative PCR and to an Agilent 2100 Bioanalyzer for DNA/RNA quantitation and quality assessment, and (4) purification and banking of patient genomic DNA. We will also provide workshops in conjunction with the Bioinformatics Core on methods central to research plans of the undergraduate research projects. All core services will be available at the start of the grant period. There is substantial need for these services among INBRE, COBRE and other investigators. It is our expectation that the Genomics Core Facility will enable INBRE and COBRE investigators to identify and characterize candidate genes involved in cancer and cardiovascular disease, provide direct evidence for experimental hypotheses, confirm patterns of gene expression, and gain insights into the physiological and biochemical functions of the candidate genes. Personnel Title D. Primerano, PhD Director J. Denvir, PhD Genomic Data Analyst G. Boskovic, PhD Microarray Manager Y. Dementieva, PhD Biostatistician Liping Wei, MS Genomics Core Manager Jun Fan, PhD Genomics Core Manager (July 1 to present) Ivana Yang, PhD Bioinformatics consultant Mark Flood, PhD Pyrosequencing Core @FSU

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 微阵列实验和自动 DNA 测序需要复杂的方法、昂贵的仪器和试剂以及密集的数据管理和分析。基因组学核心将提供小型机构无法提供的集中服务，并提供完成生物医学研究项目所需方法的培训。我们的主要目标是实现 INBRE、COBRE 以及西弗吉尼亚大学和学院的其他研究项目的基因组研究目标。在 WV-INBRE 第二阶段，基因组学核心设施将提供以下服务： (1) 基于微阵列的基因表达谱和数据分析的统计支持 (2) 与 WV-INBRE 生物信息学核心合作进行自动化 DNA 测序和使用 DNA 序列分析软件， (3) 使用实时热循环仪进行定量 PCR，使用 Agilent 2100 生物分析仪进行 DNA/RNA 定量和质量评估，以及 (4)患者基因组DNA的纯化和储存。 我们还将与生物信息学核心联合举办研讨会，讨论本科生研究项目研究计划的核心方法。所有核心服务将在资助期开始时提供。 INBRE、COBRE 和其他研究者对这些服务有很大的需求。 我们期望基因组学核心设施将使 INBRE 和 COBRE 研究人员能够识别和表征与癌症和心血管疾病有关的候选基因，为实验假设提供直接证据，确认基因表达模式，并深入了解候选基因的生理和生化功能。 人员职称 D. Primerano，博士主任 J. Denvir 博士 基因组数据分析师 G. Boskovic，博士 微阵列经理 Y. Dementieva，生物统计学博士 魏丽萍，MS Genomics 核心经理 范军，博士基因组学核心经理（7月1日至今） Ivana Yang，博士生物信息学顾问 Mark Flood 博士焦磷酸测序核心 @FSU