GENOMICS CORE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 微阵列实验和自动DNA测序需要复杂的方法、昂贵的仪器和试剂以及密集的数据管理和分析。基因组学核心将提供小型机构无法提供的集中服务，并提供完成生物医学研究项目所需方法的培训。我们的主要目标是实现INBRE、COBRE和西弗吉尼亚大学和学院的其他研究项目的基因组研究目标。根据WV-INBRE第二阶段，基因组学核心设施将提供以下服务： (1)基于微阵列的基因表达谱分析和数据分析的统计支持 (2)与WV-INBRE生物信息学核心合作，自动进行DNA测序和使用DNA序列分析软件， (3)使用实时热循环仪进行定量聚合酶链式反应，并使用安捷伦2100生物分析仪进行DNA/RNA定量和质量评估；以及 (4)患者基因组DNA的提纯和存库。 我们还将与生物信息学核心一起提供工作坊，讲授本科生研究项目研究计划的核心方法。所有核心服务将在授权期开始时提供。INBRE、Cobre和其他调查人员对这些服务的需求很大。 我们期望基因组学核心设施将使INBRE和Cobre研究人员能够识别和表征与癌症和心血管疾病有关的候选基因，为实验假说提供直接证据，确认基因表达模式，并深入了解候选基因的生理和生化功能。 人员职称 D.Primerano，博士主任 J.Denvir博士，基因组数据分析师 G.Boskovic，博士微阵列经理 Y.Dementieva，生物统计学家博士 微软基因组学核心经理魏立平 范军，博士基因组学核心经理(7月1日至今) 伊万娜·杨，生物信息学博士顾问 马克·弗洛德，博士，焦磷酸测序核心@FSU

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Microarray experimentation and automated DNA sequencing require sophisticated methods, expensive instrumentation and reagents, and intense data management and analysis. The Genomics Core will provide centralized services that would not be available to smaller institutions and provide training in methods needed for completion of biomedical research projects. Our primary objective is to enable the genomic research goals of INBRE, COBRE and other research projects at WV universities and colleges. Under WV-INBRE Phase II the Genomics Core Facility will provide the following services: (1) microarray-based gene expression profiling and statistical support for data analysis (2) automated DNA sequencing and access to DNA sequence analysis software in collaboration with the WV-INBRE Bioinformatics Core, (3) access to real-time thermal cyclers for quantitative PCR and to an Agilent 2100 Bioanalyzer for DNA/RNA quantitation and quality assessment, and (4) purification and banking of patient genomic DNA. We will also provide workshops in conjunction with the Bioinformatics Core on methods central to research plans of the undergraduate research projects. All core services will be available at the start of the grant period. There is substantial need for these services among INBRE, COBRE and other investigators. It is our expectation that the Genomics Core Facility will enable INBRE and COBRE investigators to identify and characterize candidate genes involved in cancer and cardiovascular disease, provide direct evidence for experimental hypotheses, confirm patterns of gene expression, and gain insights into the physiological and biochemical functions of the candidate genes. Personnel Title D. Primerano, PhD Director J. Denvir, PhD Genomic Data Analyst G. Boskovic, PhD Microarray Manager Y. Dementieva, PhD Biostatistician Liping Wei, MS Genomics Core Manager Jun Fan, PhD Genomics Core Manager (July 1 to present) Ivana Yang, PhD Bioinformatics consultant Mark Flood, PhD Pyrosequencing Core @FSU