Genomics Core

基因组学核心摘要 基因组学核心是一个提供基因组研究服务的集中式资源，Access 用于基因组和核酸分析仪器，并支持基因组数据的分析。这个 基因组学核心公司将提供服务，支持拟议的WV-INBRE网络及其调查人员。 在与生物信息学核心的合作中，我们建议提供所需的基因组学服务 由领队和本科生调查人员。这些服务包括(1)下一代高吞吐量 支持全基因组、全外显子组、RNA-Seq、微生物组和全局染色质的测序(NGS) 研究，(2)实验设计和基因组项目的生物信息学指导和分析，(3)获取 到DNA/RNA序列分析软件，(4)自动Sanger DNA测序和基因分型 RNA/DNA质量评估，以及(5)使用基础基因组分析中使用的共享仪器。 这些服务在拥有基因组分析专业知识的核心设施中最有效地执行。至 确保WV-INBRE调查人员可以利用NGS技术并设计健壮的 除了实验，基因组学核心还将提供基因组方法方面的培训，其中包括 关于现有和新出现的新一代测序方法的研讨会以及关于 咨询。基因组学核心业务模式包括提供全方位服务的运营计划 NGS和其他基因组服务。我们期待我们的模式和我们经验丰富的指导 工作人员将允许WV-INBRE研究人员在他们的研究中成功地采用基因组方法 生成有用的数据。从长远来看，他们的发现将导致对分子基础的理解 西弗吉尼亚州常见的慢性疾病，并最终用于这些疾病的治疗。唐纳德博士 普里梅拉诺将担任基因组学核心总监，范军博士将担任核心经理。 基因组学核心于1999年在马歇尔大学成立，并得到了联邦政府的支持 自2000年以来，一直在发放补助金。

Genomics Core Abstract The Genomics Core is a centralized resource which provides genomic research services, access to genomic and nucleic acid analysis instrumentation, and support for the analysis of genomic data. The Genomics Core will offer its services in support of the proposed WV-INBRE network and its investigators. In collaboration with the Bioinformatics Core, we propose to provide genomics services that are needed by lead and undergraduate investigators. These services include (1) high throughput next generation sequencing (NGS) to support whole genome, whole exome, RNA-Seq, microbiome and global chromatin studies, (2) experimental design and bioinformatic guidance and analysis for genomic projects, (3) access to DNA/RNA sequence analysis software, (4) automated Sanger DNA sequencing and genotyping and RNA/DNA quality assessment, and (5) access to shared instrumentation used in basic genomic analysis. These services are most efficiently executed in a core facility with expertise in genomic analysis. To insure that WV-INBRE investigators can take advantage of NGS technologies and design robust experiments, the Genomics Core will also provide training in genomic methods which will include workshops on existing and emerging next generation sequencing methods as well as one on consultation. The Genomics Core business model includes a full-service plan of operation for provision of NGS and other genomic services. We anticipate that our model and guidance from our experienced staff will allow WV-INBRE investigators to successfully adopt genomic approaches in their research and generate useful data. In the long term, their findings will lead to an understanding of the molecular basis of chronic diseases common in West Virginia and ultimately to treatments for these diseases. Dr. Donald Primerano will serve as the Genomics Core Director and Dr. Jun Fan will serve as the Core Manager. The Genomics Core was established at Marshall University in 1999 and has been supported by federal grants continuously since 2000.