MARSHALL U COBRE: GENOMICS COREP=C

马歇尔·U·科布雷：基因组学 COREP=C

• 批准号: 7610731
• 负责人: Donald Anthony Primerano
• 金额: $ 36.81万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7610731
• 关键词: Area; Candidate Disease Gene; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; DNA Sequence; DNA Sequence Analysis; Data; Data Analyses; Development; Experimental Designs; Funding; Gene Expression; Gene Expression Profiling; Genomics; Goals; Grant; Human Resources; Institution; Investigation; Malignant Neoplasms; Marshal; Measurement; Messenger RNA; Methods; Molecular; Pathway interactions; Polymerase Chain Reaction; Positioning Attribute; Reagent; Research; Research Personnel; Research Project Grants; Resources; Sequence Analysis; Signal Transduction; Source; Time; Training; United States National Institutes of Health; Universities; base; data management; expectation; experience; insight; instrumentation; novel; skills

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 该毛线应用程序中的每个研究项目都在很大程度上取决于基因表达 分析其具体目标。最常见的基因组 该应用中的方法是通过微阵列，单个mRNA进行基因表达分析 实时PCR的测量丰度以及DNA测序和序列分析。 微阵列实验和DNA测序需要昂贵的仪器，并且 试剂，复杂方法以及强烈的数据管理和分析。这些任务 最有效地由核心设施人员执行。拟议的田园项目 如果不直接访问专家基因组技术和分析，就无法完成 支持。因此，该应用的目的是实施基因组核心 将满足山cor和马歇尔大学的特定需求的设施 在基于微阵列的基因表达分析的领域进行了研究，实时PCR， 自动DNA测序和DNA序列分析。两个微阵列经理 基因组数据分析师将需要大量的外部培训，以保持空间 开发并为最终用户提供有用的分析。长期目标 基因组学核心设施是将毛毛调查员定位以发现新的分子 癌症发展的途径。我们将实现基因组研究目标 通过追求以下两个具体目的，毛病研究项目：1。 基因组学核心设施并开发微阵列数据分析功能。这 具有基因组的设施将由具有 实验设计和核心管理的完全技能。考虑到经验 在关键人员和仪器的共同导演和存在中，我们很好 准备开发基因组学核心设施。我们期望基因组学 核心设施将使Cobre和Marshall University的调查人员确定 严重参与细胞信号转导的候选基因，确认 表达，并获得对癌症分子生物学的见解。