Appalachian Center for Cellular transport in Obesity Related Disorders (ACCORD) Genomics and Bioinformatics Core

马歇尔大学基因组和生物信息学核心(GABC)将提供服务，以支持 拟议的阿巴拉契亚肥胖相关疾病细胞运输中心(ACCORD)。这只科布雷 应用程序包含五个项目，每个项目都涉及细胞转运体在开发中的作用 肥胖症。尽管它们的目标和具体目标各不相同，但所有五个项目都至少依赖于GABC中的一个 为完成其研究目标提供服务；四个调查员项目和一个合作项目将 需要RNA-Seq表达分析。GABC的目标是使基因组和生物信息学 这项Cobre申请的研究目标，并协助其他研究人员获得和分析基因组 数据。GABC将为Cobre调查人员提供以下五项服务：(1)高吞吐量NEXT 支持RNA-Seq和其他全球基因组研究的世代测序(NGS)，(2)生物信息学 NGS项目的方法和分析，(3)自动Sanger DNA测序和RNA/DNA质量 评估，(4)访问关键共享仪器，包括实时热循环仪、Luminex 200系统、 Protein Simple荧光仪和spectraMax平板阅读器以及(5)关于NGS和生物信息学的研讨会 科布雷研究项目的核心方法。GABC业务模式包括以下全方位服务计划 向科布雷调查人员提供NGS和其他基因组服务的业务。我们预计我们的 全服务模式将鼓励Cobre和其他研究人员在他们的 这些相互作用将促进西弗吉尼亚州GABC和基因组研究的增长。 GABC将使Cobre调查人员能够识别和表征有助于 解释转运蛋白在肥胖发展中的作用。我们的战略计划和集中的专业知识将 提高研究过程的效率，改进访问并减少时间和成本 基因组数据分析。参与GABC的长期影响将是刺激分子的发现 西弗吉尼亚州流行的慢性病的机制。

The Marshall University Genomics and Bioinformatics Core (GABC) will offer its services in support of the proposed Appalachian Center for Cellular Transport in Obesity Related Disorders (ACCORD). This COBRE application contains five projects each of which addresses the role of cellular transporters in the development of obesity. Although their goals and specific aims vary, all five projects rely on at least one of the GABC services for completion of their research goals; four of the investigator projects and a collaborative project will require RNA-Seq expression profiling. The objective of the GABC is to enable the genomic and bioinformatics research goals of this COBRE application and to assist other investigators in obtaining and analyzing genomic data. The GABC will provide the following five services to COBRE investigators: (1) high throughput Next Generation Sequencing (NGS) in support of RNA-Seq and other global genomic studies, (2) bioinformatic methods and analyses for NGS projects, (3) automated Sanger DNA sequencing and RNA/DNA quality assessment, (4) access to critical shared instruments including real-time thermal cyclers, Luminex 200 system, Protein Simple Fluorimeter and SpectraMax plate reader and (5) workshops on NGS and bioinformatic methods central to the COBRE research projects. The GABC business model includes a full-service plan of operation for provision of NGS and other genomic services to the COBRE investigators. We anticipate that our full service model will encourage COBRE and other investigators to adopt genomic approaches in their research and that these interactions will promote the growth of GABC and genomic research in West Virginia. The GABC will enable COBRE investigators to identify and characterize candidate pathways that help to explain the role of transporters in the development of obesity. Our strategic plan and centralized expertise will improve the efficiency of the research process in terms of improved access and reduced time and cost of genomic data analysis. The long term impact of GABC participation will be to stimulate discovery of molecular mechanisms in chronic diseases prevalent in West Virginia.