Appalachian Center for Cellular transport in Obesity Related Disorders (ACCORD) Genomics and Bioinformatics Core

阿巴拉契亚肥胖相关疾病细胞运输中心 (ACCORD) 基因组学和生物信息学核心

• 批准号: 10460403
• 负责人: Donald Anthony Primerano
• 金额: $ 13.18万
• 依托单位: MARSHALL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-02-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10460403
• 关键词: Address; Adopted; Appalachian Region; Applications Grants; Bioinformatics; Businesses; Candidate Disease Gene; Centers of Research Excellence; Chronic Disease; DNA; DNA sequencing; Data; Data Analyses; Development; Disease; Educational workshop; Elements; Experimental Designs; Expression Profiling; Funding; Genomic approach; Genomics; Goals; Grant; Growth; Human Resources; Improve Access; Individual; Methods; Modeling; Molecular; Obesity; Pathogenesis; Pathway interactions; Play; Process; Productivity; Proteins; Quality Control; RNA; Reader; Research; Research Personnel; Research Project Grants; Research Support; Role; Science; Service delivery model; Services; Strategic Planning; System; Testing; Time; Universities; West Virginia; cost; experience; gene function; genomic data; improved; insight; instrument; next generation sequencing; obesity development; operation; quality assurance; success; transcriptome sequencing

The Marshall University Genomics and Bioinformatics Core (GABC) will offer its services in support of the proposed Appalachian Center for Cellular Transport in Obesity Related Disorders (ACCORD). This COBRE application contains five projects each of which addresses the role of cellular transporters in the development of obesity. Although their goals and specific aims vary, all five projects rely on at least one of the GABC services for completion of their research goals; four of the investigator projects and a collaborative project will require RNA-Seq expression profiling. The objective of the GABC is to enable the genomic and bioinformatics research goals of this COBRE application and to assist other investigators in obtaining and analyzing genomic data. The GABC will provide the following five services to COBRE investigators: (1) high throughput Next Generation Sequencing (NGS) in support of RNA-Seq and other global genomic studies, (2) bioinformatic methods and analyses for NGS projects, (3) automated Sanger DNA sequencing and RNA/DNA quality assessment, (4) access to critical shared instruments including real-time thermal cyclers, Luminex 200 system, Protein Simple Fluorimeter and SpectraMax plate reader and (5) workshops on NGS and bioinformatic methods central to the COBRE research projects. The GABC business model includes a full-service plan of operation for provision of NGS and other genomic services to the COBRE investigators. We anticipate that our full service model will encourage COBRE and other investigators to adopt genomic approaches in their research and that these interactions will promote the growth of GABC and genomic research in West Virginia. The GABC will enable COBRE investigators to identify and characterize candidate pathways that help to explain the role of transporters in the development of obesity. Our strategic plan and centralized expertise will improve the efficiency of the research process in terms of improved access and reduced time and cost of genomic data analysis. The long term impact of GABC participation will be to stimulate discovery of molecular mechanisms in chronic diseases prevalent in West Virginia.

马歇尔大学基因组学和生物信息学核心 (GABC) 将提供服务来支持 拟议的阿巴拉契亚肥胖相关疾病细胞运输中心（ACCORD）。这个COBRE 应用程序包含五个项目，每个项目都解决细胞转运蛋白在开发中的作用 肥胖症。尽管其目标和具体目标各不相同，但所有五个项目都至少依赖于 GABC 之一 为完成研究目标提供服务；四个研究者项目和一个合作项目将 需要 RNA-Seq 表达谱分析。 GABC 的目标是使基因组和生物信息学成为可能 此 COBRE 应用程序的研究目标，并协助其他研究人员获取和分析基因组 数据。 GABC将为COBRE调查人员提供以下五项服务：（1）高通量下一步 支持 RNA-Seq 和其他全球基因组研究的一代测序 (NGS)，(2) 生物信息学 NGS 项目的方法和分析，(3) 自动 Sanger DNA 测序和 RNA/DNA 质量 评估，(4) 访问关键共享仪器，包括实时热循环仪、Luminex 200 系统， 蛋白质简单荧光计和 SpectraMax 读板机以及 (5) NGS 和生物信息学研讨会 COBRE 研究项目的核心方法。 GABC 商业模式包括以下全方位服务计划： 为 COBRE 研究人员提供 NGS 和其他基因组服务。我们预计我们的 全方位服务模式将鼓励 COBRE 和其他研究人员在他们的研究中采用基因组方法 这些相互作用将促进西弗吉尼亚州 GABC 和基因组研究的发展。 GABC 将使 COBRE 研究人员能够识别和描述候选途径，从而帮助 解释转运蛋白在肥胖发展中的作用。我们的战略计划和集中的专业知识将 提高研究过程的效率，即改善访问并减少研究时间和成本 基因组数据分析。 GABC 参与的长期影响将是刺激分子发现 西弗吉尼亚州流行的慢性疾病的机制。