EFFECT OF CIGARETTE SMOKE CONDENSATE ON FIBROBLAST VIABILITY AND APOPTOSIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 研究已经将吸烟和烟草使用与癌症的发生联系起来。 人体多器官和皮肤过早老化。我们目前正在研究香烟烟雾如何改变成纤维细胞和角质形成细胞行为的机制。 目的：为了更好地了解烟草烟雾暴露引起的人体皮肤的生理和形态变化，我们利用器官型组织工程皮肤模型(RAFT)研究了成纤维细胞活性与香烟烟雾冷凝物(CSC)的关系。 材料和方法： 用成体和新生细胞系构建的木筏分别暴露在0、10、25和50g/mLCSC中14天。测量了筏子的尺寸，以计算随时间的收缩。用多光子显微镜对胶原结构进行成像。在一个成体细胞系上，用荧光染色系统和共聚焦显微镜研究了细胞的存活率和凋亡率。 结果：本研究确定了收缩和CSC浓度之间的剂量依赖关系。CSC暴露后纤维结构也发生了变化。暴露于CSC可导致细胞凋亡和细胞死亡。 结论：CSC对排泄物具有细胞毒作用，可能与二手烟烟雾对人体皮肤的生理和形态改变有关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Studies have linked cigarette smoking and tobacco use to carcinogenesis in multiple organs of the human body and premature skin aging. We are currently investigating mechanisms of how cigarette smoke alters fibroblast and keratinocyte behavior. OBJECTIVE: In order to provide a better understanding of the physiological and morphological changes in the human skin due to tobacco smoke exposure, we examined the relationship between fibroblast activity and cigarette smoke condensate (CSC) using organotypic tissue-engineered skin models (RAFTs). MATERIALS AND METHODS: RAFTs constructed using adult and neonatal cell lines were exposed to 0, 10, 25, and 50 ¿g/mL of CSC for 14 days. RAFT dimensions were measured to calculate contraction over time. Multi-photon microscopy was also performed to image collagen structure. On an adult cell line, cell viability and apoptosis were studied using fluorescence dye systems and confocal microscopy. RESULTS: This study identified a dose-dependent relationship between contraction and CSC concentration. Fiber structure changed after CSC exposure as well. Exposure to CSC resulted in apoptosis and cell death. CONCLUSION: These findings suggest that CSC has a cytotoxic effect on RAFTs and thus fibroblast activity, which may be linked to the physiological and morphological changes that are observed in human skin exposed to second-hand cigarette smoke.