ROLE OF BREAST CANCER SUSCEPTIBILITY GENE 2 IN DNA DAMAGE REPAIR AND RESPONSE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 BRCA 2与遗传性人类乳腺癌有关。在细胞中，BRCA 2是通过同源重组途径修复DNA双链断裂所必需的。我们已经确定了一种蛋白质复合物，通过使用BRCA 2抗体进行免疫沉淀试验。BRCA 2相关伙伴之一（KIF 4A）特别有趣，因为它直接与DNA相互作用。在这项研究中，我们希望解决BRCA 2/Kif 4A复合物在DNA损伤修复和反应中的作用，这对于细胞预防癌症引发的遗传改变事件至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. BRCA2 is associated with hereditary human breast cancers. In cells, BRCA2 is required for DNA double strand break repair via the homologous recombination pathway. We have identified a protein complex by using BRCA2 antibody to perform an immunoprecipitation assay. One of the BRCA2-associating partners (KIF4A) is particuarly interesting because it directly interacts with DNA. In this study, we would like to address the role of BRCA2/Kif4A complex in DNA damage repair and response, which is critically important for cells to prevent cancer-initiating genetic alteration events.