ROLE OF BREAST CANCER SUSCEPTIBILITY GENE 2 IN DNA DAMAGE REPAIR AND RESPONSE

乳腺癌易感基因 2 在 DNA 损伤修复和反应中的作用

• 批准号: 7722582
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.67万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722582
• 关键词: Address; Antibodies; BRCA2 gene; Biological Assay; Cancer-Predisposing Gene; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Double Strand Break; DNA Repair; Double Strand Break Repair; Event; Funding; Grant; Human; Immunoprecipitation; Inherited; Institution; Malignant Neoplasms; Mutation; Pathway interactions; Proteins; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; homologous recombination; interest; malignant breast neoplasm; prevent; repaired; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. BRCA2 is associated with hereditary human breast cancers. In cells, BRCA2 is required for DNA double strand break repair via the homologous recombination pathway. We have identified a protein complex by using BRCA2 antibody to perform an immunoprecipitation assay. One of the BRCA2-associating partners (KIF4A) is particuarly interesting because it directly interacts with DNA. In this study, we would like to address the role of BRCA2/Kif4A complex in DNA damage repair and response, which is critically important for cells to prevent cancer-initiating genetic alteration events.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 BRCA2 与遗传性人类乳腺癌有关。在细胞中，BRCA2 是通过同源重组途径修复 DNA 双链断裂所必需的。我们通过使用 BRCA2 抗体进行免疫沉淀测定来鉴定蛋白质复合物。 BRCA2 相关伙​​伴之一 (KIF4A) 特别有趣，因为它直接与 DNA 相互作用。在这项研究中，我们希望探讨 BRCA2/Kif4A 复合物在 DNA 损伤修复和反应中的作用，这对于细胞预防癌症引发的基因改变事件至关重要。