MOUSE BRAIN GLYCOPROTEINS EXPRESSING O-MAN AND ASN-LINKED GLYCANS

表达 O-MAN 和 ASN 连接聚糖的小鼠脑糖蛋白

• 批准号: 7722628
• 负责人: J. Michael Pierce
• 金额: $ 0.38万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722628
• 关键词: Brain; Cell Adhesion; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Funding; Glycoproteins; Grant; Institution; Knockout Mice; Link; Mannose; Mass Spectrum Analysis; Mus; Neurons; Polysaccharides; Research; Research Personnel; Resources; Source; Techniques; United States National Institutes of Health; brain tissue; glycosyltransferase; migration; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A relatively new type of O-linked glycan has been implicated in neuronal cell adhesion and migration, the O-linked mannose glycans. This project will use novel techniques to separate glycoproteins expressing O-Man glycans from brain tissue and identify these glycoproteins using mass spectrometry techniques. In addition, the Asn-linked glycans from mouse brain have been identified by MALDI-TOF MS. These are being compared to glycans found in mice that are null for the GnT-Va glycosyltransferase to determine how this enzyme regulates glycoprotein glycan expression. A GnT-Vb null mouse has now been produced and the effects of this deletion on O-Man and Asn-linked glycoproteins is now under study.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 一种相对新型的O-连接聚糖与神经细胞粘附和迁移有关， 甘露糖聚糖 该项目将使用新技术分离表达O-Man聚糖的糖蛋白， 并使用质谱技术鉴定这些糖蛋白。此外，Asn连接的 已经通过MALDI-TOF MS鉴定了来自小鼠脑的聚糖。 GnT-Va糖基转移酶无效的小鼠，以确定该酶如何调节糖蛋白聚糖 表情 现在已经产生了GnT-Vb无效小鼠，并且这种缺失对O-Man和Asn连锁的 糖蛋白目前正在研究中。