BREAST CARCINOMA GLYCOPROTEIN CHANGES CAUSED BY ONCOGENE EXPRESSION

癌基因表达引起的乳腺癌糖蛋白变化

• 批准号: 7722620
• 负责人: J. Michael Pierce
• 金额: $ 0.38万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-08 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7722620
• 关键词: Binding; Biological Tumor Markers; Biopsy; Breast; Breast Carcinoma; Cells; Computer Retrieval of Information on Scientific Projects Database; Funding; Glycoproteins; Grant; Human; Institution; Isoelectric Focusing; Label; Mammary Gland Parenchyma; Membrane; Messenger RNA; N-Acetylglucosaminyltransferases; N-Acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase; Oncogenes; Pattern; Plant Lectins; Research; Research Personnel; Resources; Source; Techniques; Tissues; United States National Institutes of Health; glycosylation; neoplastic; polyvinylidene fluoride; tumor progression; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Expression of the human oncogene, her-2, causes selective increases in N-acetylglucosaminyltransferase T-V (GlcNAc T-V) mRNA and activity. Histological studies of breast tissue using a plant lectin that binds to the product of GlcNAc T-V show that the product is absent from non-diseased breast tissue, but is expressed when cells appear neoplastic. The GlcNAc T-V product increases in expression during tumor progression. This project focuses on identifying particular glycoproteins in breast biopsy tissue that express the GlcNAc T-V product in order to define possible biochemical tumor markers for breast carcinoma. To identify these glycoproteins, two-dimensional isoelectric focusing/SDS-PAGE techniques and LC-Q-TOF techniques are being used. After separation and electroblotting to PVDF membranes, labeled plant lectins are used to identify unique glycosylation patterns on the blotted glycoproteins.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人癌基因her-2的表达导致N-乙酰葡糖胺转移酶T-V（GlcNAc）的选择性增加 T-V）mRNA和活性。使用与GlcNAc产物结合的植物凝集素进行乳腺组织的组织学研究 T-V显示该产物不存在于非患病乳腺组织中，但在细胞出现肿瘤时表达。 GlcNAc T-V产物在肿瘤进展期间表达增加。该项目的重点是确定 乳腺活检组织中表达GlcNAc T-V产物的特定糖蛋白，以确定可能的 乳腺癌的生化肿瘤标志物。为了鉴定这些糖蛋白，二维等电 使用聚焦/SDS-PAGE技术和LC-Q-TOF技术。分离和电印迹后， PVDF膜，标记的植物凝集素用于鉴定印迹上的独特糖基化模式。 糖蛋白