VARIATIONS IN SECOND HARMONIC AND TWO PHOTON FLUORESCENCE SIGNALS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 组织中的胶原纤维和原纤维直径和取向在疾病过程中可能变化很大，例如哮喘和慢性阻塞性肺病，其特征在于气道胶原沉积和瘢痕形成。胶原微结构的病理变化会影响细胞外基质的力学性能，这可能会影响细胞行为和总体组织和器官的力学性能。通过系统地改变胶原蛋白水凝胶的微观结构，基本的组织工程支架，我们可以改变水凝胶的机械性能和线性和非线性散射性能在一个可控的方式。本研究的目的是将胶原蛋白水凝胶光学性质（二次谐波产生，SHG和双光子荧光，TPF）的变化与聚合条件范围内的水凝胶机械性质的变化相关联。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Collagen fiber and fibril diameter and orientation in tissue can vary greatly in disease processes such as asthma and chronic obstructive pulmonary disorder, which are characterized by airway collagen deposition and scar formation. Pathological changes in collagen microstructure affect mechanical properties of extracellular matrix, which may affect both cell behavior and gross tissue and organ mechanical properties. By systematically varying the microstructure of collagen hydrogels, basic tissue engineering scaffolds, we can change both hydrogel mechanical properties and linear and nonlinear scattering properties in a controlled manner. The purpose of this study is to correlate changes in collagen hydrogel optical properties (second harmonic generation, SHG, and two-photon fluorescence, TPF) with changes in hydrogel mechanical properties over a range of polymerization conditions.