ROLE OF BREAST CANCER SUSCEPTIBILITY GENE 2 IN DNA DAMAGE REPAIR AND RESPONSE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 BRCA2与遗传性人类乳腺癌有关。在细胞中，BRCA2是DNA双链断裂修复所必需的同源重组途径。我们通过使用BRCA2抗体进行免疫沉淀实验鉴定了一种蛋白质复合体。BRCA2结合伙伴之一(KIF4A)特别有趣，因为它直接与DNA相互作用。在这项研究中，我们想要探讨BRCA2/Kif4A复合体在DNA损伤修复和反应中的作用，这对细胞预防癌症启动的基因改变事件至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. BRCA2 is associated with hereditary human breast cancers. In cells, BRCA2 is required for DNA double strand break repair via the homologous recombination pathway. We have identified a protein complex by using BRCA2 antibody to perform an immunoprecipitation assay. One of the BRCA2-associating partners (KIF4A) is particuarly interesting because it directly interacts with DNA. In this study, we would like to address the role of BRCA2/Kif4A complex in DNA damage repair and response, which is critically important for cells to prevent cancer-initiating genetic alteration events.