ADSC/HUVEC VASCULARIZED FIBRIN

ADSC/HUVEC 血管化纤维蛋白

• 批准号: 7954842
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.09万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954842
• 关键词: Adipose tissue; Basement membrane; Biotechnology; Blood capillaries; Color; Computer Retrieval of Information on Scientific Projects Database; Differentiation Antigens; Endothelial Cells; Fibrin; Fibroblasts; Funding; Grant; Human; Hydrogels; Immunofluorescence Immunologic; Institution; Lasers; Lung; Membrane Proteins; Pericytes; Proteins; Research; Research Personnel; Resources; Source; Stem cells; Umbilical vein; United States National Institutes of Health; Viral Vector; capillary; cell type; human stem cells; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human adipose-derived stem cells (AdSCs) and human umbilical vein endothelial cells (HUVECs) will be cultured in a 3D fibrin hydrogel and allowed to form capillaries. Previous experiments have shown that human mesechymal stem cells and lung fibroblasts have associated in a pericytic fashion with the newly form capillaries. This project hopes to show that the AdSCs also associate in a pericyte-like manner to help stabilize newly formed capillaries. This will be done by fluorescently tagging the different cell types with viral vectors encoding a colored protein of choice or also through immunofluorescence to tag for differentiation markers. Basement membrane proteins will also be screened for via immunofluorescence as another sign of mature capillaries.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 人脂肪干细胞(AdSCs)和人脐静脉内皮细胞(HUVECs)将在3D纤维蛋白水凝胶中培养，并允许形成毛细血管。以前的实验表明，人类间充质干细胞和肺成纤维细胞与新形成的毛细血管以周细胞方式联系在一起。该项目希望表明，AdSCs也以周细胞样的方式结合，以帮助稳定新形成的毛细血管。这将通过用编码所选有色蛋白的病毒载体荧光标记不同类型的细胞来完成，或者也可以通过免疫荧光来标记分化标记。基底膜蛋白也将通过免疫荧光进行筛选，作为成熟毛细血管的另一个标志。