OCT AND MPM CHARACTERIZATION OF RABBIT TRACHEAL SCRAPE INJURY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室研究由上皮细胞引起的气道纤维化 损伤，这是哮喘和其他疾病的共同特征。在 为了扩展我们的组织工程体外模型， 刮伤导致上皮下纤维化，并发展为OCT 和MPM作为一种潜在的临床工具， 在纤维化疾病期间监测气道重塑，我们希望 刮取兔气管上皮， (up至1周）上皮下富含胶原蛋白层的变化， 使用内窥镜OCT和传统MPM。我们还将比较 刮取与未刮取气管粘膜的机械性能， 并将机械性能的变化与 粘膜的胶原蛋白和弹性蛋白微观结构，如所示 通过多光子图像。所有动物研究将在 根据批准的UCI动物方案#2007-2754。的副本 将向BLI提供批准的方案和任何修正案。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab studies fibrosis in airways caused by epithelial injury, which is a common feature of asthma and other diseases. In order to extend our tissue-engineered in vitro models of epithelial scrape injury leading to subepithelial fibrosis, and to develop OCT and MPM as a potential clinical tool for characterizing and monitoring remodeling in airways during fibrotic diseases, we wish to scrape rabbit tracheal epithelium and track short and longer-term (up to 1 week) changes in the subepithelial collagen-rich layer, using endoscopic OCT and conventional MPM. We will also be comparing the mechanical properties of scraped vs. unscraped tracheal mucosa, and correlating changes in the mechanical properties to changes in the collagen and elastin microstructure of the mucosa, as revealed by multiphoton images. All animal studies will be conducted in accordance with approved UCI animal protocol #2007-2754. A copy of the approved protocol and any amendments will be provided to BLI.