REGULATION OF N-GLYCOSYLATION BY STIMULATION OF THE UNFOLDED PROTEIN RESPONSE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 参与糖核苷酸互变、N-甲基-N- 聚糖脂质连接的寡糖和参与未折叠蛋白质反应的蛋白质正在通过qRT定量。 PCR法 Lehrman实验室的重点是未折叠蛋白质反应和N-聚糖结构的变化 脂质连接寡糖加工途径的干扰。 人类培养的细胞将被处理与各种 诱导未折叠蛋白应答和转录物变化的试剂将与LLO聚糖库的变化相关。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcript levels for various enzymes and proteins involved in interconversion of sugar nucleotides, biosynthesis of N- glycan lipid-linked oligosaccharides, and proteins involved in the unfolded protein response are being quantitated by qRT- PCR. The focus of the Lehrman laboratory is on unfolded protein response and changes in N-glycan structures as a result of perturbation of the lipid-linked oligosaccharide processing pathway. Human cultured cells will be treated with various agents to induce the unfolded protein response and transcript changes will be correlated with changes in LLO glycan pools.