ENDOCRINOLOGY LABORATORY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在过去的一年里，ART核心为辅助生殖技术(ART)股以及TNPRC的另外两个PIS进行了区域影响评估。其中包括5项雌二醇测定和454项皮质醇测定。RIA检测使用了从诊断产品公司购买的试剂盒。这些试剂盒包括标准品、I125标记的激素和与特定抗体结合的试管。 该试验是一种竞争性结合试验。结果是根据与试管结合的标记荷尔蒙的量来确定的，并与标准进行比较。使用TNPRC开发的计算机程序来建立最佳拟合曲线，并根据所分析的样本数量，在分析后6至48小时内将结果提供给调查人员。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the past year the ART Core has conducted RIAs for the Assisted Reproductive Technology (ART) Unit as well as for two other PIs at the TNPRC. These included 5 assays for estradiol and 454 assays to determine cortisol levels. RIA assays utilized kits that were purchased from Diagnostic Products Corporation. These kits include standards, I125 labeled hormone, and tubes bound with specific antibody. The assay is a competitive binding assay. Results were determined based on the amount of labeled hormone bound to the tubes and compared to the standards. A computer program developed at the TNPRC was used to establish as "best fit" curve and results were provided to the investigators within 6 to 48 hours of the assay, depending on the number of samples assayed.