Core C: Structural Imaging Core

这项建议的中心主题是剖析导致组织损伤和器官损伤的分子机制 创伤中的功能障碍。消息、蛋白质或结构变化的可视化和本地化 在这一动态过程中的每一步所产生的结果对于理解 精神创伤。这项提案中的不同项目侧重于明确的组织系统，包括肠道、肝脏和肺 在休克和/或创伤模型中。在每一种情况下，都将使用广泛的微观方法和 被认为是在组织和细胞水平上了解HS的病理学所必需的，因此 中央细胞和组织成像核心被定义为该提案的组成部分。核心将被安置在 在匹兹堡大学医学中心的生物成像中心(CBL)。这个中心配备了 执行光学方法的连续体，包括本计划项目所必需的所有类型的显微镜。 在本计划的范围内，光学显微镜技术包括：组织学、免疫组织学、激光 共聚焦、双光子、消逝波、活细胞和整体/动物成像技术。我们可观的 具备计算机图像处理和形态测量经验，能够对观察到的数据进行定量分析 现象，以证实微妙的质的变化，这是核心在本计划的主要功能。在… 电子显微镜水平的薄层电子显微镜和免疫电子显微镜评价 当需要时，将采用标本作为光学显微镜分析的自然延伸。在.期间 在上一个赠款周期中，CBL与所有项目负责人进行了广泛的合作，如 初步数据部分，我们预计光学技术的使用将继续扩大。此外，我们 开发和构建了多个新工具，以促进从单一的 细胞传给整个动物 相关性(请参阅说明)： 光学成像是提供基于图像的定量数据的程序中的基本核心功能 使用计算机辅助工具进行的后续分析也可在核心内使用

The central theme of this proposal is to dissect the molecular mechanisms leading to tissue injury, and organ dysfunction in trauma. Visualization and localization of message, protein, or structural changes leading to, or resulting from each step in this dynamic process is essential in understanding the molecular mechanisms of trauma. The different projects within this proposal focus on defined tissue systems including gut, liver and lung within models of shock and or trauma. In each case a wide range of microscopic methods will be employed and are considered essential in gaining an understanding the pathology of HS at the tissue and cellular level, thus a central cell and tissue imaging core is defined as an integral component of this proposal. The Core will be housed in the Center for Biologic Imaging (CBl) of the University of Pittsburgh Medical Center. This Center is equipped to perform a continuum of optical methods including all types of microscopy essential to this Program Project. Within the scope of this Program light microscopic techniques include: histological, immuno-histological, laser confocal, 2 photon, evanescent wave, live cell and whole dssue/animal imaging technologies. Our considerable experience in computerized image processing and morphometry will allow quantitative analysis of observed phenomena to corroborate subtle qualitative changes, and this a major function of the Core in this Program. At the electron microscopic level thin section electron microscopy and immuno-electron microscopic evaluation of specimens as a natural extension of the light microscopic analyses will be employed when needed. During the previous grant cycle the CBl collaborated extensively with all of project leaders, as is described in the preliminary data section and we expect a continued expansion in the use of optical techniques. Furthermore we have developed and built multiple new instruments to facilitate these interactions at all levels from the single cell to the whole animal RELEVANCE (See Instructions): Optical imaging is an essential core function within the program providing quantitative image based data for subsequent analysis using computer aided tools also available within the core