Cell and Tissue Imaging Core

细胞和组织成像核心

• 批准号: 7663829
• 负责人: SIMON C WATKINS
• 金额: $ 6.21万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7663829
• 关键词: Anatomy; Cells; Cellular biology; Confocal Microscopy; Core Facility; Data; Depth; Disease; Duchenne muscular dystrophy; Dystrophin; Effectiveness; Electron Microscopy; Electrons; Evaluation; Gene Delivery; Gene Expression; Gene Transfer; Genes; Goals; Histology; Human Resources; Image; Image Analysis; Imagery; Imaging Device; Imaging technology; Immunologics; Knowledge; Label; Lasers; Life; Light; Locales; Maintenance; Messenger RNA; Methods; Microscopic; Mind; Muscle; Optical Methods; Optics; Pathology; Phenotype; Proteins; Range; Research; Research Personnel; Resolution; Resources; Role; Scientist; Services; Skeletal Muscle; Specimen; Stem cells; Structural Protein; System; Techniques; Technology; Therapeutic; Tissues; Training; Treatment Protocols; Universities; cellular pathology; computerized; design; experience; image processing; immunocytochemistry; instrumentation; intracellular protein transport; light microscopy; medical schools; morphometry; novel; programs; protein localization location; research study; vector

Visualization and localization of message, protein, or structural change resulting from gene expression is an essential step in evaluating the efficacy of successful gene transfer into cells and tissues. The goals of this program are to extend our understanding of the cellular pathology of Duchenne Muscular Dystrophy and to develop vector systems for dystrophin delivery using a variety of possible delivery systems, including AAV, Herpes and stem cells. It is expected that these vectors will be used to use these vectors to generate clinically useful therapeutic regimens. In each case there is a fundamental need to define the level of incorporation of the delivered dystrophin into muscle, and to assess the effectiveness of the therapy. This identification varies from the low resolution studies of whole tissues, defining correction of pathological phenotype, to high resolution observations of successful subcellular passaging and presentation of protein. The Center for Biologic Imaging, in which this core service will be performed, is designed with this function in mind. It is equipped to perform a continuum of optical methods including all types of light and electron microscopy essential to this program project. Within the scope of this project at the light microscopic level these include: histological, immuno-histological and possibly live cell technologies. At the electron microscopic level we will provide fine structural and immuno-electron microscopic evaluation of specimens as a natural extension of the light microscopic analyses when needed. Furthermore, our considerable experience in computerized image processing and morphometry will allow quantitative analysis of observed _henomena to corroborate earlier, possibly quite subtle qualitative changes. This core will be used extensively by all projects, though the imaging tools used will vary from project to project. Preliminary data have shown the validity of these approaches, and we expect a very significant increase in the use of optical techniques within the formal setting of this program

可视化和定位信息，蛋白质，或基因表达引起的结构变化是一个重要的研究领域。 这是评估成功将基因转移到细胞和组织中的功效的重要步骤。这个的目标 计划是为了扩大我们对杜氏肌营养不良症的细胞病理学的理解， 开发用于肌营养不良蛋白递送的载体系统，使用多种可能的递送系统，包括AAV， 疱疹和干细胞。预计这些载体将用于使用这些载体生成 临床上有用的治疗方案。在每一种情况下，都有一个基本的需要， 将递送的肌营养不良蛋白掺入肌肉，并评估治疗的有效性。这 识别不同于整个组织的低分辨率研究，定义了病理学的校正。 表型，以高分辨率观察成功的亚细胞传代和蛋白质的呈递。 生物成像中心将执行此核心服务，其设计具有此功能， 主意了它配备了一个连续的光学方法，包括所有类型的光和电子 显微镜对这个项目至关重要。在本项目的范围内，在光学显微镜水平上， 这些技术包括：组织学、免疫组织学和可能的活细胞技术。在电子 显微镜水平，我们将提供精细的结构和免疫电镜评价标本， 在需要时，光学显微镜分析的自然延伸。此外，我们的大量 在计算机图像处理和形态测量的经验将允许定量分析观察到的 - -现象证实了早先可能相当微妙的质的变化。这个核心将用于 所有项目都广泛使用，尽管所使用的成像工具因项目而异。初步数据 我们已经证明了这些方法的有效性，我们预计在使用光学 在这个程序的正式设置中的技术