REGULATION OF N-GLYCOSYLATION BY STIMULATION OF THE UNFOLDED PROTEIN RESPONSE

通过刺激未折叠蛋白反应来调节 N-糖基化

• 批准号: 8170736
• 负责人: KELLEY W. MOREMEN
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170736
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Enzymes; Funding; Grant; Human; Institution; Laboratories; Nucleotide Biosynthesis; Pathway interactions; Polysaccharides; Process; Proteins; Regulation; Research; Research Personnel; Resources; Source; Structure; Transcript; United States National Institutes of Health; glycosylation; lipooligosaccharide; response; sugar nucleotide

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Transcript levels for various enzymes and proteins involved in interconversion of sugar nucleotides, biosynthesis of N-glycan lipid-linked oligosaccharides, and proteins involved in the unfolded protein response are being quantitated by qRT-PCR. The focus of the Lehrman laboratory is on unfolded protein response and changes in N-glycan structures as a result of perturbation of the lipid-linked oligosaccharide processing pathway. Human cultured cells will be treated with various agents to induce the unfolded protein response and transcript changes will be correlated with changes in LLO glycan pools.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 通过 qRT-PCR 可以对参与糖核苷酸相互转化、N-聚糖脂联寡糖生物合成以及参与未折叠蛋白反应的蛋白质的转录本水平进行定量。 莱尔曼实验室的重点是由于脂质连接寡糖加工途径的扰动而导致的未折叠蛋白质反应和 N-聚糖结构的变化。 人类培养的细胞将用各种试剂处理以诱导未折叠的蛋白质反应，并且转录物的变化将与 LLO 聚糖库的变化相关。