DEVELOPMENT OF HIGH-PERFORMANCE DIGITAL IMAGING SYSTEMS FOR MESOSCALE IVEM APPS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 为中尺度IVEM应用开发高性能数字成像系统。 这项工作的重点是技术、研究和开发，以改进数字成像技术，作为胶片的替代品，并作为高通量、广域中尺度数据采集和计算机集成显微镜的使能技术。具体地说，我们正在改进8k x 8k超广域镜头耦合ccd相机系统的使用，用于显微镜生产。同样，我们正在开展工程活动，将新出现的滴滴涕原型整合到我们的资源IVEM上进行常规数据采集。作为这些系统持续开发和改进的驱动力，我们培育了利用这些设备的性能特征的中尺度显微镜应用程序，并将它们应用于合作研究。这些目标具体分为以下几个目标：第一，为适应超广域高通量图像采集的需要，我们将完成NCMIR 8k x 8k镜头耦合CCD相机系统的集成和调试。第二，为了更准确地记录生物标本的信息，我们将改进技术，使用NCMIR发明的抗辐射CMOS相机直接记录图像，并将这些DDD放在关键资源平台上。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DEVELOPMENT OF HIGH-PERFORMANCE DIGITAL IMAGING SYSTEMS FOR MESOSCALE IVEM APPLICATIONS. This effort focuses on technology, research, and development to improve technologies for digital imaging as a replacement for film and as an enabling technology for high-throughput, wide-field mesoscale data acquisition and computer-integrated microscopy. Specifically, we are refining use of a 8k x 8k ultra-wide-field lens-coupled CCD camera system for production use for microscopy. Similarly, we are undertaking engineering activities to integrate emerging DDD prototypes for routine data acquisition on our resource IVEMs. As a driver for the continued development and refinement of these systems, we cultivate mesoscale microscopy applications that exploit the performance characteristics of these devices and apply them to collaborative research. These goals are organized into the following specific aims: first, to accommodate the need for ultra-wide-field high-throughput image acquisition, we will complete the integration and commissioning of the NCMIR 8k x 8k lens-coupled CCD camera system. Second, to more accurately record information about biological specimens, we will refine technologies to directly record images using radiation-hardened CMOS cameras invented at NCMIR and field these DDDs on key resource platforms.