Structural Analysis of Supramolecular Assemblies by Hybrid Methods

描述（由申请人提供）：请求对由Andrej Sali、Brian T.查特和大卫贝克。会议将于2013年3月3日至7日在加州的太浩城举行。为了了解活细胞的运作，我们需要知道单个生物大分子的结构和动力学，它们的复合体和网络，细胞器和整个细胞，从而将结构生物学与细胞生物学，系统生物学，蛋白质组学，化学生物学和生物物理学联系起来。结构表征的准确性、精确度、完整性和效率通过混合或综合方法最大化，所述混合或综合方法通过使用计算分析和建模方法将来自多种类型的实验方法的数据联合收割机组合。这些实验方法包括X射线晶体学、NMR光谱、电子显微镜、小角X射线散射、质谱、化学交联、超高分辨率光学显微镜、光学显微镜等。本次会议的目标是突出目前的混合方法的优势和局限性，以及确定新的有前途的进展，收集数据的实验方法和计算方法，将数据转换为模型。最后，混合方法将通过其在重要和具有挑战性的生物学问题中的应用来证明，这些问题跨越了广泛的长度和时间尺度，从单个蛋白质的原子结构到整个细胞的低分辨率表示。

DESCRIPTION (provided by applicant): Support is requested for a Keystone Symposia meeting entitled Structural Analysis of Supramolecular Assemblies by Hybrid Methods, organized by Andrej Sali, Brian T. Chait and David Baker. The meeting will be held in Tahoe City, California from March 3 - 7, 2013. In order to understand the workings of a living cell, we need to know the structures and dynamics of individual biological macromolecules, their complexes and networks, organelles, and whole cells, thus interfacing structural biology with cell biology, systems biology, proteomics, chemical biology, and biophysics. The accuracy, precision, completeness, and efficiency of structural characterization are maximized by hybrid or integrative approaches that combine data from multiple types of experimental methods through the use of computational analysis and modeling methods. These experimental methods include X-ray crystallography, NMR spectroscopy, electron microscopy, small angle X- ray scattering, mass spectrometry, chemical cross-linking, super-high resolution optical microscopy, optical microscopy, and others. The goal of this meeting is to highlight the strengths and limitations of the current hybrid approaches as well as to identify new promising advances in experimental methods for collecting the data and computational methods for converting the data to models. Finally, hybrid approaches will be demonstrated by their applications to important and challenging biological problems, spanning a wide range of length and time scales, from atomic structures of individual proteins to low-resolution representations of whole cells.