WaNPRC Macaca nemestrina SPF Breeding Colony

描述（由申请人提供）：拟议项目的目标是维持和扩大猪尾猕猴（Macaca nemestrina）的无特定病原体（SPF）繁殖群，以支持华盛顿国家灵长类动物研究中心（WaNPRC）的NIH支持研究。猕猴是人类疾病的宝贵模型，也是生物医学研究的重要资源。M.奈米司季纳具有独特的特性，使其非常宝贵，特别是在艾滋病发病机制、免疫学、疫苗开发和阴道杀微生物剂评价的研究中。WaNPRC保持着M唯一的主要国内繁殖群体。奈美丝蒂娜为了满足对M.近年来，由于WaNPRC的nemestrina，有必要从印度尼西亚进口动物。即使进口，满足NIH支持的研究动物的要求也是一个挑战。我们建议在未来四年内将我们的国内SPF繁殖群扩大到1400只动物的自我维持普查，这将满足对M的预期需求。奈美丝蒂娜我们将采取两项主要战略来满足这一需求：改进管理和进口育种动物。M.奈梅斯特里纳不像其他猕猴品种那样哈代，并且有不同的繁殖要求。我们最近建立了一个繁殖设施的M。亚利桑那州的nemestrina由WaNPRC直接管理，并专门关注该物种。我们预计，通过这种策略，我们将能够降低死亡率并提高生育率，从而使该群体中的动物数量净增加。我们还将进口总计200 M。nemestrina来达到我们的殖民扩张目标。我们将在装运前对进口动物进行病毒抗体血清学检测，并通过内部重复血清学检测确认其无病原体状态。干扰研究结果（SIV、STLV、SRV）或对人类具有人畜共患病风险（McHV-1，也称为疱疹B）的病毒病原体将通过将未感染的后代与感染的祖先分开饲养并定期检测殖民地中的所有动物，从殖民地中消除。

DESCRIPTION (provided by applicant): The goal of the proposed project is to maintain and expand a specific-pathogen free (SPF) breeding colony of pigtail macaques (Macaca nemestrina) for the support of NlH-supported research at the Washington National Primate Research Center (WaNPRC). Macaques are a valuable model of human disease and are an essential resource for biomedical research. M. nemestrina possess unique characteristics that make them invaluable, particularly in the study of AIDS pathogenesis, immunology, vaccine development, and vaginal microbicide evaluation. The WaNPRC maintains the only major domestic breeding colony of M. nemestrina. In order to meet the demand for M. nemestrina at the WaNPRC in recent years, it has been necessary to import animals from Indonesia. Even with the importation, it has been a challenge to meet requests for animals for NlH-supported research. We propose over the course of the next four years to expand our domestic SPF breeding colony to a self-sustaining census of 1400 animals which will meet the anticipated demand for M. nemestrina. We will employ two major strategies to meet this demand: Improved management, and importation of breeding animals. M. nemestrina are not as hardy as other macaque species, and have different breeding requirements. We have recently established a breeding facility for M. nemestrina in Arizona that is directly managed by the WaNPRC, and focuses specifically on that species. We anticipate that with this strategy we will be able to reduce mortality and increase fertility, resulting in a net increase in the number of animals in th colony. We will also import a total of 200 M. nemestrina to reach our goal of colony expansion. We will have imported animals tested serologically for viral antibodies prior to shipment and confirm their pathogen-free status with in-house repeated serologic testing. Viral pathogens that interfere with research results (SIV, STLV, SRV) or are a zoonotic risk to humans (McHV-1, also known as herpes B) will be eliminated from the colony by raising uninfected offspring separately from infected progenitors and testing all animals in the colony regularly.