REDUCING ANNEXIN I OR II IN 3-D CULTURE BENIGN PROSTATIC EPITHELIAL CELLS

减少 3D 培养良性前列腺上皮细胞中的附件蛋白 I 或 II

• 批准号: 7597865
• 负责人: TATIANA B KRASIEVA
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7597865
• 关键词: Annexin A1; Annexins; Architecture; Benign; Cell Polarity; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Epithelial Cells; Funding; Grant; Institution; Laser Scanning Confocal Microscopy; Lesion; Link; Microscopy; Optical Coherence Tomography; Premalignant; Prostatic; Prostatic Intraepithelial Neoplasias; Proteins; Research; Research Personnel; Resources; Source; Structure; System; Technology; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It is generally accepted that high-grade prostatic intraepithelial neoplasia (HGPIN) is a precancerous lesion. Several groups, including ours, have demonstrated that the majority of HGPIN lesions have reduced or absent levels of annexins (ANX) I and II proteins. However, a causative link between the reduced expression of annexins and HGPIN has not been established. To investigate this possibility we have silenced ANX I or II expression in benign prostatic epithelial cells and examined the glandular architecture and cellular polarity in 3-dimentional (3-D) culture. To determine if silencing ANX I and II expression in normal prostatic epithelial cells alters development of acinar structures in 3-D culture, we will investigate 3-D structures by using confocal laser scanning microscopy (LSM), multiphoton microscopy (MPM) and MPM/optical coherence tomography (OCT) system. Preliminary data obtained with LAMMP technologies were used for NIH grant submission.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 高级别前列腺上皮内瘤变（HGPIN）是一种癌前病变。 包括我们在内的几个研究小组已经证明，大多数HGPIN病变的膜联蛋白（ANX）I和II蛋白水平降低或缺失。 然而，尚未建立膜联蛋白和HGPIN表达减少之间的因果关系。 为了研究这种可能性，我们沉默了良性前列腺上皮细胞中ANX I或II的表达，并在三维（3-D）培养中检查了腺体结构和细胞极性。为了确定正常前列腺上皮细胞中ANX I和II表达的沉默是否会改变3-D培养中腺泡结构的发育，我们将通过使用共聚焦激光扫描显微镜（LSM），多光子显微镜（MPM）和MPM/光学相干断层扫描（OCT）系统研究3-D结构。 使用LAMMP技术获得的初步数据用于NIH拨款提交。