REDUCING ANNEXIN I OR II IN 3-D CULTURE BENIGN PROSTATIC EPITHELIAL CELLS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 人们普遍认为高级别前列腺上皮内瘤变(HGPIN)是一种癌前病变。包括我们在内的几个小组已经证明，大多数HGPIN病变的膜联蛋白(ANX)I和II蛋白水平降低或缺失。然而，膜联蛋白表达减少与HGPIN之间的因果关系尚未确定。为了研究这种可能性，我们在良性前列腺上皮细胞中沉默了ANX I或II的表达，并在三维培养中检测了腺体的结构和细胞的极性。为了确定在三维培养中抑制正常前列腺上皮细胞ANX I和II的表达是否改变腺泡结构的发育，我们将使用激光共聚焦扫描显微镜(LSM)、多光子显微镜(MPM)和MPM/光学相干断层扫描(OCT)系统来研究三维结构。 使用LAMP技术获得的初步数据被用于NIH拨款提交。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. It is generally accepted that high-grade prostatic intraepithelial neoplasia (HGPIN) is a precancerous lesion. Several groups, including ours, have demonstrated that the majority of HGPIN lesions have reduced or absent levels of annexins (ANX) I and II proteins. However, a causative link between the reduced expression of annexins and HGPIN has not been established. To investigate this possibility we have silenced ANX I or II expression in benign prostatic epithelial cells and examined the glandular architecture and cellular polarity in 3-dimentional (3-D) culture. To determine if silencing ANX I and II expression in normal prostatic epithelial cells alters development of acinar structures in 3-D culture, we will investigate 3-D structures by using confocal laser scanning microscopy (LSM), multiphoton microscopy (MPM) and MPM/optical coherence tomography (OCT) system. Preliminary data obtained with LAMMP technologies were used for NIH grant submission.