ANALYSIS OF B-WELL MOM SAMPLES FY 2019

背景和导言 呼吸-健康，环境，生活方式和肺功能研究（B-WELL母亲研究）的目的是检查哮喘控制在妊娠结局中的作用。哮喘症状在怀孕期间会发生变化，约1/3的女性症状改善，1/3的女性症状恶化。因此，该研究在妊娠免疫学和遗传、环境和社会健康决定因素等风险因素的背景下，对哮喘控制不良进行了研究。 在怀孕的前三个月（<14周）招募患有哮喘和没有哮喘的妇女，并随访至产后4个月。评估发生在每个妊娠期（<14周，20-22周，30-32周），分娩时和产后4个月。收集母亲哮喘状态、孕前哮喘控制、特应性状态、肺功能、炎症标志物、人体测量学、行为和人口统计学信息，并与妊娠结局和婴儿人体测量学一起进行评估。主要关注的暴露包括过敏、免疫功能和妊娠期及产后肺部炎症。 本项目的主要目标是探索特应性状态是否预测哮喘控制变异性;评估特应性状态是否与肺功能和炎症变化相关;评估Treg和Foxp 3与哮喘控制变异性之间的关系;以及在炎症标志物、空气污染物和饮食抗氧化剂之间的关联背景下评估肺功能和炎症。 B-WELL Mom研究在每次访问（<14周，20- 22周，30-32周）和产后4个月时从大约400名受试者收集尿液，微生物组拭子和血液样品（PAXGene RNA，血清，血浆和血沉棕黄层）。在妊娠期间采集一次鼻拭子，同时在分娩时采集胎盘和脐带血。脐带血RNA在递送时直接收集到PAXGene中。 范围 本研究的目的是探索特应性状态是否能预测哮喘控制的变异性（目的1）;评估特应性状态和哮喘症状是否决定了免疫相关基因的表达模式（目的2）;以及确定哮喘状态是否反映在炎症标志物的水平上（目的3）。

BACKGROUND AND INTRODUCTION The purpose of the Breathe – Wellbeing, Environment, Lifestyle and Lung Function Study (B-WELL Mom Study) is to examine the role of asthma control in pregnancy outcomes. Asthmasymptoms change during pregnancy, with about 1/3 of women improving and 1/3 of womengetting worse. As such, the study examines poor asthma control in the context of theimmunology of pregnancy and risk factors such as genetics, the environment, and socialdeterminants of health. Women with and without asthma were recruited during the first trimester of pregnancy (< 14weeks gestation) and followed through 4 months post-partum. Assessments occurred duringeach trimester of pregnancy (<14 weeks, 20-22 weeks, 30-32 weeks), at delivery, and 4 monthspost-partum. Maternal asthma status, pre-pregnancy control of asthma, atopy status, lungfunction, inflammatory markers, anthropometrics, behaviors, and demographic informationwere collected and assessed alongside pregnancy outcomes and infant anthropometrics. Primaryexposures of interest included allergies, immune function, and lung inflammation duringpregnancy and postpartum. The primary goals of this project are to explore if atopy status predicts asthma controlvariability; to assess if atopy status is associated with changes in lung function andinflammation; to evaluate the relationship between Treg and Foxp3 on asthma controlvariability; and to evaluate lung function and inflammation in the context of associationsbetween inflammatory markers, air pollutants, and dietary antioxidants. The B-WELL Mom study collected urine, microbiome swabs, and blood samples (PAXGene RNA,serum, plasma, and buffy coat) from approximately 400 subjects at each visit (<14 weeks, 20-22weeks, 30-32 weeks) and 4 months post-partum. Nasal swabs were collected once duringpregnancy while placentas, and cord blood were taken at delivery. Cord blood RNA was collectedat delivery directly into PAXGene. SCOPE The objective of this task order is to explore if atopy status predicts asthma control variability(Aim 1); to assess if atopy status and asthma symptoms dictate patterns of expression ofimmune-related genes (Aim 2); and to determine if asthma status is reflected in the level ofinflammatory markers (Aim 3).