BIOMEDICAL ASSAY LABORATORY FOR THE DIVISION OF INTRAMURAL POPULATION HEALTH RESEARCH

校内人口健康研究部（DIPHR）对与人类生殖和发育、儿童和青少年健康和行为以及孕妇护理等有关的各种主题进行研究。 观察性和干预性研究都是由DIPHR科学家设计和实施的，大多数研究包括收集各种数据和生物标本。 所有生物标本均采用经批准的方案收集，并在存入NICHD支持的储存库之前进行去识别。 承包商不得收到任何关于研究受试者的身份或个人健康信息。 采集的标本类型广泛，包括全血、脐带血、血沉棕黄层、血浆、血清、尿液、唾液、组织（如胎盘、网膜脂肪）、毛发、指甲剪下物、卵泡液和精液、精液和新生儿血斑。 DIPHR需要分析多种标本类型的多种因素，包括生殖激素和其他激素的浓度;饮食生物标志物;细胞因子;代谢功能指标，包括分析;用于表达阵列的RNA分离;和蛋白质组分析。 DIPHR需要分析采集的生物样本，包括血液（血浆、血清、WBC等），尿液、唾液、脂肪组织、卵泡液、精液和其他与特定研究计划相关的生物材料。 建议的分析包括类固醇激素浓度的测量;总IgE和过敏原特异性IgE的测定;细胞分选的T细胞亚群的表达阵列分析;从全血和血斑中提取DNA;测量尿白三烯;从唾液中纯化DNA;鉴定天然存在的植物雌激素和代谢物;使用多峰和ELISA技术测量不同分析物的灵活性;以及代谢组学、蛋白质组学和全基因组关联研究的定量。

The division of Intramural Population Health Research (DIPHR) conducts research on a variety of topics pertaining to human reproduction and development, child and adolescent health and behavior, and care of pregnant women, among other topics. Both observational and interventional research studies are designed and are implemented by DIPHR scientists, and most research includes collection of a diverse array of data and bio specimens. All bio specimens are collected using approved protocols and are de-identified prior to banking in NICHD-supported repositories. The contractor shall receive no identifying or personal health information about the study subjects. A wide range of specimen types are collected including whole blood, cord blood, buffy coats, plasma, serum, urine, saliva, tissues (e.g. placenta, omental fat), hair, nail clippings, follicular and seminal fluid, semen, and new born blood spots. DIPHR requires analysis of this diverse collection of specimen types for a multitude of factors, including concentrations of reproductive and other hormones; dietary biomarkers; cytokines; indicators of metabolic function including analysis; RNA isolation for use in expression arrays; and proteome analysis. DIPHR requires analysis of collected biological specimens including blood (plasma, serum, WBC, etc.), urine, saliva, adipose tissue, follicular fluid, seminal fluid, and other biologic material relevant for specific research initiatives. Proposed analyses include measurement of concentrations of steroid hormones; assay of total and allergen-specific IgE; expression array analysis of cell-sorted T cell subpopulations; extraction of DNA from whole blood and blood spots; measurement of urinary leukotrienes; purification of DNA from saliva; identification of naturally occurring phytoestrogens and metabolites; the flexibility to measure different analytes using multipex and ELISA techniques; and quantification of metabolomics, proteomics, and genome-wide association studies.